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Journal of Clinical Microbiology, April 1999, p. 1221-1223, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Production of Specific Monoclonal Antibodies to Aspergillus Species and Their Use in Immunohistochemical Identification of Aspergillosis

L. E. Fenelon,1,* A. J. Hamilton,2 J. I. Figueroa,2 M. A. Bartholomew,2 M. H. Allen,2 P. McCarthy,1 and R. J. Hay2

Departments of Clinical Microbiology and Histopathology, St. Vincent's Hospital, Dublin 4, Ireland,1 and Dermatology Unit, St. John's Institute of Dermatology, Guy's Hospital, Kings College, London SE1 9RT, United Kingdom2

Received 10 August 1998/Returned for modification 25 September 1998/Accepted 9 January 1999

Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining.

* Corresponding author. Mailing address: Departments of Clinical Microbiology and Histopathology, St. Vincent's Hospital, Elm Park, Dublin 4, Ireland. Phone: 353-1-2094470. Fax: 353-1-2094112. E-mail: lfenelon{at}svherc.ucd.ie.

Journal of Clinical Microbiology, April 1999, p. 1221-1223, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chaturvedi, A. K., Kavishwar, A., Shiva Keshava, G. B., Shukla, P. K. (2005). Monoclonal Immunoglobulin G1 Directed against Aspergillus fumigatus Cell Wall Glycoprotein Protects against Experimental Murine Aspergillosis. CVI 12: 1063-1068 [Abstract] [Full Text]  


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