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Journal of Clinical Microbiology, April 1999, p. 1224-1226, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Specificity of IS6110-Based DNA Fingerprinting and Diagnostic Techniques for Mycobacterium tuberculosis Complex

Willie A. Githui,1,2,3,* Stuart M. Wilson,1 and Francis A. Drobniewski1

PHLS Mycobacterium Reference Unit, Dulwich Public Health Laboratory and Department of Microbiology, Kings College School of Medicine and Dentistry, Dulwich Hospital,1 and Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine,2 London, United Kingdom; and Centre for Respiratory Diseases Research, Kenya Medical Research Institute, Nairobi, Kenya3

Received 4 September 1998/Returned for modification 3 November 1998/Accepted 1 January 1999

Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific primers yielded a 181-bp fragment only in DNA from M. tuberculosis complex isolates.

* Corresponding author. Mailing address: PHLS Mycobacterium Reference Unit, Dulwich Public Health Laboratory and Department of Microbiology, Kings College School of Medicine and Dentistry, Kings College Hospital (Dulwich), East Dulwich Grove, London SE22 8QF, United Kingdom. Phone: 44 (0) 181-693-2830. Fax: 44 (0) 171-346-6477. E-mail: w.githui{at}lshtm.ac.uk.

Journal of Clinical Microbiology, April 1999, p. 1224-1226, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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