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Journal of Clinical Microbiology, May 1999, p. 1480-1483, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of E-Test and Broth Microdilution Methods for Antifungal Drug Susceptibility Testing of Molds

Adrien Szekely, Elizabeth M. Johnson,* and David W. Warnock

Mycology Reference Laboratory, Public Health Laboratory Service, Bristol, United Kingdom

Received 4 December 1998/Returned for modification 26 January 1999/Accepted 18 February 1999

We compared the E test with a broth microdilution method, performed according to National Committee for Clinical Laboratory Standards document M27-A guidelines, for determining the in vitro susceptibilities of 90 isolates of pathogenic molds (10 Absidia corymbifera, 10 Aspergillus flavus, 10 Aspergillus fumigatus, 10 Aspergillus niger, 10 Aspergillus terreus, 10 Exophiala dermatitidis, 10 Fusarium solani, 10 Scedosporium apiospermum, 5 Scedosporium prolificans, and 5 Scopulariopsis brevicaulis). Overall, there was 71% agreement between the results of the two methods for amphotericin B (E-test MICs within ±2 log2 dilutions of broth microdilution MICs) and 88% agreement with the results for itraconazole. The overall levels of agreement (within ±2 log2 dilutions) were >= 80% for 5 of the 10 species tested against amphotericin B and 8 of the 10 species tested against itraconazole. The best agreement between the results was seen with A. fumigatus and A. terreus (100% of results for both agents within ±2 log2 dilutions). The poorest agreement was seen with S. apiospermum, S. prolificans, and S. brevicaulis tested against amphotericin B (20% of results within ±2 log2 dilutions). In every instance, this low level of agreement was due to isolates for which the broth microdilution MICs were low but for which the E-test MICs were much higher. The E test appears to be a suitable alternative procedure for testing the susceptibility of Aspergillus spp. and some other molds to amphotericin B or itraconazole.

* Corresponding author. Mailing address: Mycology Reference Laboratory, Public Health Laboratory, Kingsdown, Bristol BS2 8EL, United Kingdom. Phone: (44) 117-928-5031. Fax: (44) 117-922-6611.

Journal of Clinical Microbiology, May 1999, p. 1480-1483, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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