This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Doglio, A.
Right arrow Articles by Lefebvre, J.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doglio, A.
Right arrow Articles by Lefebvre, J.-C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 1999, p. 1567-1569, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Second Generation of the Automated Cobas Amplicor HCV Assay Improves Sensitivity of Hepatitis C Virus RNA Detection and Yields Results That Are More Clinically Relevant

Alain Doglio,1,* Catherine Laffont,1 François X. Caroli-Bosc,2 Pascale Rochet,1 and J.-Claude Lefebvre1

Laboratoire de Virologie, Faculté de Médecine, 06107 Nice,1 and Service d'Hépato-Gastroentérologie, Hôpital de l'Archet II, 06202 Nice,2 France

Received 19 October 1998/Returned for modification 6 January 1999/Accepted 29 January 1999

The first and second generations of the Cobas Amplicor HCV assay were compared among patients at risk of hepatitis C virus (HCV) infection. The second-generation test was found to be of greater sensitivity and of good specificity among clinical specimens containing HCV RNA of different genotypes. Finally, this new test is shown to predict the outcome of interferon therapy better.

* Corresponding author. Mailing address: Laboratoire de Virologie, Faculté de Médecine, Avenue de Valombrose, 06107 Nice Cedex 2, France. Phone: (33)04.93.37.76.78. Fax: (33)04.93.81.54.84. E-mail: doglio{at}unice.fr.

Journal of Clinical Microbiology, May 1999, p. 1567-1569, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Barbeau, J. M., Goforth, J., Caliendo, A. M., Nolte, F. S. (2004). Performance Characteristics of a Quantitative TaqMan Hepatitis C Virus RNA Analyte-Specific Reagent. J. Clin. Microbiol. 42: 3739-3746 [Abstract] [Full Text]  
  • Krajden, M., Ziermann, R., Khan, A., Mak, A., Leung, K., Hendricks, D., Comanor, L. (2002). Qualitative Detection of Hepatitis C Virus RNA: Comparison of Analytical Sensitivity, Clinical Performance, and Workflow of the Cobas Amplicor HCV Test Version 2.0 and the HCV RNA Transcription-Mediated Amplification Qualitative Assay. J. Clin. Microbiol. 40: 2903-2907 [Abstract] [Full Text]  
  • Trimoulet, P., Halfon, P., Pohier, E., Khiri, H., Chene, G., Fleury, H. (2002). Evaluation of the VERSANT HCV RNA 3.0 Assay for Quantification of Hepatitis C Virus RNA in Serum. J. Clin. Microbiol. 40: 2031-2036 [Abstract] [Full Text]  
  • Stelzl, E., Kormann-Klement, A., Haas, J., Daghofer, E., Santner, B. I., Marth, E., Kessler, H. H. (2002). Evaluation of an Automated Sample Preparation Protocol for Quantitative Detection of Hepatitis C Virus RNA. J. Clin. Microbiol. 40: 1447-1450 [Abstract] [Full Text]  
  • Nolte, F. S., Fried, M. W., Shiffman, M. L., Ferreira-Gonzalez, A., Garrett, C. T., Schiff, E. R., Polyak, S. J., Gretch, D. R. (2001). Prospective Multicenter Clinical Evaluation of AMPLICOR and COBAS AMPLICOR Hepatitis C Virus Tests. J. Clin. Microbiol. 39: 4005-4012 [Abstract] [Full Text]  
  • Sarrazin, C., Hendricks, D. A., Sedarati, F., Zeuzem, S. (2001). Assessment, by Transcription-Mediated Amplification, of Virologic Response in Patients with Chronic Hepatitis C Virus Treated with Peginterferon {alpha}-2a. J. Clin. Microbiol. 39: 2850-2855 [Abstract] [Full Text]  
  • Kessler, H. H., Stelzl, E., Raggam, R. B., Haas, J., Kirchmeir, F., Hegenbarth, K., Daghofer, E., Santner, B. I., Marth, E., Stauber, R. E. (2001). Effects of Storage and Type of Blood Collection Tubes on Hepatitis C Virus Level in Whole Blood Samples. J. Clin. Microbiol. 39: 1788-1790 [Abstract] [Full Text]  
  • Ratge, D., Scheiblhuber, B., Nitsche, M., Knabbe, C. (2000). High-Speed Detection of Blood-borne Hepatitis C Virus RNA by Single-Tube Real-Time Fluorescence Reverse Transcription-PCR with the LightCycler. Clin. Chem. 46: 1987-1989 [Full Text]  
  • Lee, S. C., Antony, A., Lee, N., Leibow, J., Yang, J. Q., Soviero, S., Gutekunst, K., Rosenstraus, M. (2000). Improved Version 2.0 Qualitative and Quantitative AMPLICOR Reverse Transcription-PCR Tests for Hepatitis C Virus RNA: Calibration to International Units, Enhanced Genotype Reactivity, and Performance Characteristics. J. Clin. Microbiol. 38: 4171-4179 [Abstract] [Full Text]  
  • Yu, M.-L., Chuang, W.-L., Dai, C.-Y., Chen, S.-C., Lin, Z.-Y., Hsieh, M.-Y., Wang, L.-Y., Chang, W.-Y. (2000). Clinical Evaluation of the Automated COBAS AMPLICOR HCV MONITOR Test Version 2.0 for Quantifying Serum Hepatitis C Virus RNA and Comparison to the Quantiplex HCV Version 2.0 Test. J. Clin. Microbiol. 38: 2933-2939 [Abstract] [Full Text]  
  • Kleiber, J., Walter, T., Haberhausen, G., Tsang, S., Babiel, R., Rosenstraus, M. (2000). Performance Characteristics of a Quantitative, Homogeneous TaqMan RT-PCR Test for HCV RNA. J. Mol. Diagn. 2: 158-166 [Abstract] [Full Text]  
  • Martinot-Peignoux, M., Le Breton, V., Fritsch, S., Le guludec, G., Labouret, N., Keller, F., Marcellin, P. (2000). Assessment of Viral Loads in Patients with Chronic Hepatitis C with AMPLICOR HCV MONITOR Version 1.0, COBAS HCV MONITOR Version 2.0, and QUANTIPLEX HCV RNA Version 2.0 Assays. J. Clin. Microbiol. 38: 2722-2725 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»