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Journal of Clinical Microbiology, May 1999, p. 1582-1583, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

False-Positive Results Obtained with the Alexon ProSpecT Cryptosporidium Enzyme Immunoassay

Kirk M. Doing,1,2,* Jill L. Hamm,3 Jo Ann Jellison,2 Jessica A. Marquis,2 and Cindy Kingsbury2

Department of Microbiology, Biochemistry and Molecular Biology, University of Maine, Orono, Maine 044691; Affiliated Laboratory, Inc., Bangor, Maine 044012; and Togus Veterans Administration Center and Hospital, Togus, Maine 043303

Received 27 October 1998/Returned for modification 7 December 1998/Accepted 4 February 1999

Cryptosporidium is known to cause diarrhea in immunocompromised patients and is also associated with outbreaks of disease due to food-borne and waterborne parasites. Traditional procedures, involving iodine staining of wet mounts of stool sediments and trichrome staining, lack the sensitivity to detect Cryptosporidium. Special staining procedures, such as the modified acid-fast and safranin stains, are generally employed. Less labor-intensive antigen detection assays have simplified detection; however, careful attention to local epidemiology is important because false-positive tests occur. Here, we report two incidents involving 62 false-positive results obtained with the Alexon ProSpecT Cryptosporidium enzyme immunoassay, which were deemed false-positive based on negative results obtained from extensive microscopic examinations.

* Corresponding author. Mailing address: Affiliated Laboratory, Inc., 925 Union St., Suite 4, Bangor, ME 04401. Phone: (207) 973-6900. Fax: (207) 973-6999. E-mail: doing{at}maine.maine.edu.

Journal of Clinical Microbiology, May 1999, p. 1582-1583, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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