This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Badak, F. Z.
Right arrow Articles by Bilgic, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Badak, F. Z.
Right arrow Articles by Bilgic, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 1999, p. 1602-1605, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Use of Nucleic Acid Probes for Identification of Mycobacterium tuberculosis Directly from MB/BacT Bottles

F. Zuhre Badak,1,* Servet Goksel,1 Ruchan Sertoz,1 Bedii Nafile,2 Safak Ermertcan,1 Cengiz Cavusoglu,1 and Altinay Bilgic1

Department of Clinical Microbiology1 and Department of Infectious Diseases,2 Ege University Medical School, Izmir, Turkey

Received 30 September 1998/Returned for modification 4 November 1998/Accepted 20 January 1999

The feasibility of using nucleic acid probes directly from positive MB/BacT broth to identify mycobacteria was determined in this study. A total number of 2,727 specimens were cultured into the MB/BacT (Organon Teknika) automated system and on conventional Loweinstein-Jensen (LJ) slants. The Gen-Probe AccuProbe culture identification tests (DNA probes) were used on samples from bottles which were identified as positive for mycobacteria by MB/BacT. Samples of positive MB/BacT broth (0.1 ml) were used directly in the broth culture method for the DNA probes as published by Gen-Probe. Centrifugation of the contents of the bottle was not done prior to probe testing. The number of mycobacteria detected by MB/BacT and LJ was 253 (221 isolates of M. tuberculosis and 32 isolates of mycobacteria other than M. tuberculosis [MOTT]). A total of 96.4% (213 of 221) of the bottles growing M. tuberculosis produced a positive direct DNA probe result for M. tuberculosis complex. One hundred percent (16 of 16) of the bottles growing M. gordonae produced a positive direct DNA probe result for M. gordonae. A total of 3.6% (8 of 221) of the bottles growing M. tuberculosis did not yield a positive direct DNA probe result for M. tuberculosis complex. The testing of subcultures made onto solid media from the positive bottles by AccuProbe identified six of these eight M. tuberculosis isolates. Two (0.9%) M. tuberculosis isolates gave a negative result for the M. tuberculosis probe test applied on the MB/BacT broth and its subculture. The rest of the positive MB/BacT bottles growing MOTT (16 of 32) were negative for M. gordonae, M. avium, M. intracellulare, and M. kansasii probes. The sensitivity and specificity of AccuProbe for the identification of M. tuberculosis and M. gordonae directly from MB/BacT broth were 96.4 and 100% for M. tuberculosis and 100 and 100% for M. gordonae, respectively. The direct testing of positive MB/BacT broth by AccuProbe, without prior centrifugation, allows for the accurate and rapid identification of M. tuberculosis and M. gordonae.

* Corresponding author. Present address: 201 Harrison St., #425, San Francisco, CA 94105. Phone: (415) 546-0460. Fax: (415) 278-6666. E-mail: zarinc{at}juno.com.

Journal of Clinical Microbiology, May 1999, p. 1602-1605, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Miller, N., Cleary, T., Kraus, G., Young, A. K., Spruill, G., Hnatyszyn, H. J. (2002). Rapid and Specific Detection of Mycobacterium tuberculosis from Acid-Fast Bacillus Smear-Positive Respiratory Specimens and BacT/ALERT MP Culture Bottles by Using Fluorogenic Probes and Real-Time PCR. J. Clin. Microbiol. 40: 4143-4147 [Abstract] [Full Text]  
  • Scarparo, C., Piccoli, P., Rigon, A., Ruggiero, G., Nista, D., Piersimoni, C. (2001). Direct Identification of Mycobacteria from MB/BacT Alert 3D Bottles: Comparative Evaluation of Two Commercial Probe Assays. J. Clin. Microbiol. 39: 3222-3227 [Abstract] [Full Text]  
  • Louro, A. P. S., Waites, K. B., Georgescu, E., Benjamin, W. H. Jr. (2001). Direct Identification of Mycobacterium avium Complex and Mycobacterium gordonae from MB/BacT Bottles Using AccuProbe. J. Clin. Microbiol. 39: 570-573 [Abstract] [Full Text]  
  • Piersimoni, C., Scarparo, C., Callegaro, A., Tosi, C. P., Nista, D., Bornigia, S., Scagnelli, M., Rigon, A., Ruggiero, G., Goglio, A. (2001). Comparison of MB/BacT ALERT 3D System with Radiometric BACTEC System and Lowenstein-Jensen Medium for Recovery and Identification of Mycobacteria from Clinical Specimens: a Multicenter Study. J. Clin. Microbiol. 39: 651-657 [Abstract] [Full Text]  
  • Miller, N., Infante, S., Cleary, T. (2000). Evaluation of the LiPA MYCOBACTERIA Assay for Identification of Mycobacterial Species from BACTEC 12B Bottles. J. Clin. Microbiol. 38: 1915-1919 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»