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Journal of Clinical Microbiology, May 1999, p. 1646-1650, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cytolethal Distending Toxin Genes in Campylobacter jejuni and Campylobacter coli Isolates: Detection and Analysis by PCR

Aysegul Eyigor,1 Karl A. Dawson,1 Bruce E. Langlois,1 and Carol L. Pickett2,*

Department of Animal Sciences, College of Agriculture, University of Kentucky, Lexington, Kentucky 40546-0215,1 and Department of Microbiology and Immunology, College of Medicine, University of Kentucky, Lexington, Kentucky 40536-02982

Received 21 July 1998/Returned for modification 28 September 1998/Accepted 5 February 1999

Campylobacter jejuni produces a toxin called cytolethal distending toxin (CDT). Knowledge of the prevalence and homogeneity of Campylobacter sp. cdt genes is incomplete. In this work, we identified four PCR primer pairs that collectively amplified cdt genes in all of the C. jejuni and Campylobacter coli strains tested. Restriction analyses of the cdt PCR products showed clear differences between the cdt genes of these two species, yet there were few heterogeneities noted between members of the same species. Consequently, it may be possible to speciate C. jejuni and C. coli isolates on the basis of restriction patterns within their cdt genes.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Chandler Medical Center, 800 Rose St., University of Kentucky, Lexington, KY 40536-0298. Phone: (606) 323-5313. Fax: (606) 257-8994. E-mail: cpicket{at}pop.uky.edu.

Journal of Clinical Microbiology, May 1999, p. 1646-1650, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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