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Journal of Clinical Microbiology, June 1999, p. 1676-1682, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Multisite Reproducibility of Results Obtained by the Broth Microdilution Method for Susceptibility Testing of Mycobacterium abscessus, Mycobacterium chelonae, and Mycobacterium fortuitum

Gail L. Woods,1,* John S. Bergmann,1 Frank G. Witebsky,2 Gary A. Fahle,2 Audrey Wanger,3 Betty Boulet,3 Marianne Plaunt,4 Barbara A. Brown,5 and Richard J. Wallace Jr.5

Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-07401; Microbiology Service, Clinical Pathology Department, W. G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 208922; Department of Pathology, University of Texas---Houston Medical School, Houston, Texas 770303; StatProbe, Ann Arbor, Michigan 481084; and Department of Microbiology, University of Texas Health Center at Tyler, Tyler, Texas 757105

Received 17 December 1998/Returned for modification 6 February 1999/Accepted 27 February 1999

A multicenter study was conducted to assess the interlaboratory reproducibility of broth microdilution testing of the more common rapidly growing pathogenic mycobacteria. Ten isolates (four Mycobacterium fortuitum group, three Mycobacterium abscessus, and three Mycobacterium chelonae isolates) were tested against amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, imipenem, sulfamethoxazole, and tobramycin (M. chelonae only) in four laboratories. At each site, isolates were tested three times on each of three separate days (nine testing events per isolate) with a common lot of microdilution trays. Agreement among MICs (i.e., mode ± 1 twofold dilution) varied considerably for the different drug-isolate combinations and overall was best for cefoxitin (91.7 and 97.2% for one isolate each and 100% for all others), followed by doxycycline, amikacin, and ciprofloxacin. Agreement based on the interpretive category, using currently suggested breakpoints, also varied and overall was best for doxycycline (97.2% for one isolate and 100% for the rest), followed by ciprofloxacin and clarithromycin. Reproducibility among MICs and agreement by interpretive category was most variable for imipenem. Based on results reported from the individual sites, it appears that inexperience contributed significantly to the wide range of MICs of several drugs, especially clarithromycin, ciprofloxacin, and sulfamethoxazole. New interpretive guidelines are presented for the testing of M. fortuitum against clarithromycin; M. abscessus and M. chelonae against the aminoglycosides; and all three species against cefoxitin, doxycycline, and imipenem.

* Corresponding author. Mailing address: Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0740. Phone: (409) 772-4851. Fax: (409) 772-5683. E-mail: gwoods{at}utmb.edu.

Journal of Clinical Microbiology, June 1999, p. 1676-1682, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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