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Journal of Clinical Microbiology, June 1999, p. 2034-2036, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Oxacillin Susceptibility Testing of Staphylococci Directly from Bactec Plus Blood Cultures by the BBL Crystal MRSA ID System

M. Kubina, B. Jaulhac,* X. Delabranche, C. Lindenmann, Y. Piemont, and H. Monteil

Institut de Bactériologie, Faculté de Médecine et Hôpitaux Universitaires de Strasbourg, 67000 Strasbourg, France

Received 17 August 1998/Returned for modification 28 December 1998/Accepted 20 March 1999

The BBL Crystal MRSA ID test (Becton Dickinson) was applied directly to blood culture vials containing clusters of gram-positive cocci. The sensitivity and specificity of the test were 84 and 100% and 54 and 100% for vials containing Staphylococcus aureus and coagulase-negative staphylococci, respectively. This test is a reliable method for direct detection of methicillin resistance in positive blood culture vials when S. aureus is identified in parallel by rapid identification procedures.


* Corresponding author. Mailing address: Institut de Bactériologie, 3, rue Koeberlé, 67000 Strasbourg, France. Phone: (33) 3.88.21.19.70. Fax: (33) 3.88.25.11.13. E-mail: benoit.jaulhac{at}medecine.u-strasbg.fr.


Journal of Clinical Microbiology, June 1999, p. 2034-2036, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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  • Maes, N., Magdalena, J., Rottiers, S., De Gheldre, Y., Struelens, M. J. (2002). Evaluation of a Triplex PCR Assay To Discriminate Staphylococcus aureus from Coagulase-Negative Staphylococci and Determine Methicillin Resistance from Blood Cultures. J. Clin. Microbiol. 40: 1514-1517 [Abstract] [Full Text]