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Journal of Clinical Microbiology, June 1999, p. 2037-2039, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

One-Step Reverse Transcriptase PCR Method for Detection of Borrelia burgdorferi mRNA in Mouse Lyme Arthritis Tissue Samples

F. X. Limbach,1,2 B. Jaulhac,1,* Y. Piémont,1 J. L. Kuntz,2 H. Monteil,1 and J. Sibilia2

Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur et Hôpitaux Universitaires de Strasbourg,1 and Service de Rhumatologie, Hôpitaux Universitaires de Strasbourg,2 67000 Strasbourg, France

Received 17 August 1998/Returned for modification 14 December 1998/Accepted 17 March 1999

A one-step reverse transcriptase PCR (RT-PCR) method for detection of Borrelia burgdorferi mRNA in infected C3H mice is described. This simple procedure, less prone to nucleic acid cross-contamination than the standard method, was found to be 10-fold more sensitive than a classical two-step RT-PCR assay. By using one-step RT-PCR, flagellin mRNAs were detected in synovial and heart tissues from all seven infected mice tested.

* Corresponding author. Mailing address: Institut de Bactériologie, 3 rue Koeberlé, 67000 Strasbourg, France. Phone: 33.388.21.19.76. Fax: 33.388.25.11.13. E-mail: benoit.jaulhac{at}medecine.u-strasbg.fr.

Journal of Clinical Microbiology, June 1999, p. 2037-2039, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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