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Journal of Clinical Microbiology, June 1999, p. 2071-2073, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Epidemiological Analysis of Sequential Pseudomonas aeruginosa Isolates from Chronic Bronchiectasis Patients without Cystic Fibrosis

I. Pujana,1,* L. Gallego,1 G. Martín,2 F. López,1 J. Canduela,1 and R. Cisterna1,3

Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco,1 Servicio de Microbiología, Hospital de Santa Marina,2 and Servicio de Microbiología, Hospital de Basurto,3 Bilbao, Spain

Received 13 October 1998/Returned for modification 14 January 1999/Accepted 2 March 1999

PCR fingerprinting was used for the epidemiological investigation of 64 Pseudomonas aeruginosa isolates collected from 16 chronic bronchiectasis patients without cystic fibrosis: 56% of the patients harbored one clone, 12.5% carried a single major type with minor variants, and 31.5% carried two clones. Only a minority of the acquisitions of antibiotic resistance was related to the acquisition of exogenous strains. Mucoid and nonmucoid sets of isolates did not display any consistent differences in their patterns. The genetic similarity among the clones ranged from 10 to 69%. Cross-infection or common-source exposure did not appear to have occurred.


* Corresponding author. Mailing address: Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco, Aptdo. 699, 48080 Bilbao, Spain. Phone: 34 946012778. Fax: 34 946012869. E-mail: oibpuuri{at}lg.ehu.es.


Journal of Clinical Microbiology, June 1999, p. 2071-2073, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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