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Journal of Clinical Microbiology, June 1999, p. 2087-2089, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation of DNA after Extraction of RNA To Detect the Presence of Borrelia burgdorferi and Expression of Host Cellular Genes from the Same Tissue Sample

Kei Amemiya,1 Henry Schaefer,2 and Andrew R. Pachner2,*

National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892,1 and Department of Neurology, Georgetown University School of Medicine, Washington, D.C. 200072

Received 10 November 1998/Returned for modification 14 January 1999/Accepted 18 March 1999

We are investigating the neuropathogenesis of Lyme disease caused by Borrelia burgdorferi in a nonhuman primate model. In the past, two separate pieces of tissue had to be used when both analyzing for the presence of the spirochete and examining the host response to infection. We have modified a procedure to purify DNA from the same sample after the extraction of RNA. The remaining material containing the DNA was precipitated, and residual organic reagent was removed prior to deproteinization and extraction of the DNA. This procedure now allows us to both assay for the presence of the Lyme microorganism and analyze the host response in the same tissue preparation.

* Corresponding author. Present address: Department of Neurosciences, Bldg. MSB H506, University of Medicine and Dentistry of New Jersey, 185 S. Orange Ave., Newark, NJ 07103. Phone: (973) 972-7407. Fax: (973) 972-5059. E-mail: pachner{at}UMDNJ.edu.

Journal of Clinical Microbiology, June 1999, p. 2087-2089, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pachner, A. R., Dail, D., Li, L., Gurey, L., Feng, S., Hodzic, E., Barthold, S. (2002). Humoral Immune Response Associated with Lyme Borreliosis in Nonhuman Primates: Analysis by Immunoblotting and Enzyme-Linked Immunosorbent Assay with Sonicates or Recombinant Proteins. CVI 9: 1348-1355 [Abstract] [Full Text]  


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