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Journal of Clinical Microbiology, June 1999, p. 2090-2092, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Clinically Relevant Genotypes of Vancomycin-Resistant Enterococci in Nosocomial Surveillance Specimens by PCR

P. Jayaratne1,2,* and C. Rutherford2

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada L8N 3Z5,1 and Microbiology Section, Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences Corporation, General Campus, Hamilton, Ontario, Canada L8L 2X22

Received 2 December 1998/Returned for modification 22 January 1999/Accepted 17 March 1999

This study evaluated a PCR method for the rapid detection of clinically significant genotypes of vancomycin-resistant enterococci (VRE) in nosocomial surveillance specimens. Detection of the vanA and vanB genes by multiplex PCR using 657 specimens that showed presumptive growth of VRE on bile esculin azide agar containing 6 mg of vancomycin/liter was compared to the conventional method. The diagnostic values for the PCR compared to the phenotypic method were as follows: 99.8% specificity, 95.4% sensitivity, 98.8% positive predictive value, and 99.3% negative predictive value. The average cost per test for PCR is $8.26, compared to $9.45 for the phenotypic method. The average turnaround time for detecting a VRE is 48 h for PCR, compared to 96 h for the conventional method.

* Corresponding author. Mailing address: Microbiology Section, Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences Corporation, General Campus, 237 Barton St. East, Hamilton, Ontario, Canada, L8L 2X2. Phone: (905) 527-4322, ext. 6182. Fax: (905) 577-8027. E-mail: Jayaratn{at}FHS.McMaster.CA.

Journal of Clinical Microbiology, June 1999, p. 2090-2092, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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