This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lukinmaa, S.
Right arrow Articles by Siitonen, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lukinmaa, S.
Right arrow Articles by Siitonen, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 1999, p. 2176-2182, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Salmonella Enteritidis Phage Types 1 and 4: Pheno- and Genotypic Epidemiology of Recent Outbreaks in Finland

Susanna Lukinmaa,1 Raili Schildt,2 Teemu Rinttilä,1 and Anja Siitonen1,*

Laboratory of Enteric Pathogens, National Public Health Institute, FIN-00300 Helsinki,1 and Department of Bacteriology, National Veterinary and Food Research Institute, FIN-00580 Helsinki,2 Finland

Received 2 November 1998/Returned for modification 14 January 1999/Accepted 23 March 1999

In the 1990s, Salmonella enterica subsp. enterica serovar Enteritidis has caused 15 outbreaks in Finland; 12 of them were caused by phage type 1 (PT1) and PT4. Thus far, there has been no clear evidence as to the source of these Salmonella Enteritidis PT1 and PT4 strains, so it was necessary to try to characterize them further. Salmonella Enteritidis PT1 (n = 57) and PT4 (n = 43) isolates from different sources were analyzed by genomic pulsed-field gel electrophoresis (PFGE), plasmid profiling, and antimicrobial resistance testing to investigate the distribution of their subtypes in Finland. It was also hoped that this investigation would help in identifying the sources of the infections, especially the sources of the outbreaks caused by PT1 and PT4 in the 1990s. The results showed that both PFGE and plasmid profiling, but not antimicrobial susceptibility testing, were capable of differentiating isolates of Salmonella Enteritidis PT1 and PT4. By genotypic methods, it was possible to divide both PT1 and PT4 isolates into 12 subtypes. It could also be shown that all PT1 outbreak isolates were identical and, at least with this collection of isolates, that the outbreaks did not originate from the Baltic countries or from Russia, where this phage type predominates. It was also established that the outbreaks caused by PT4 all had different origins. Valuable information for future investigations was gained on the distribution of molecular subtypes of strains that originated from the tourist resorts that are popular among Finns and of strains that were isolated from livestock.

* Corresponding author. Mailing address: National Public Health Institute, Laboratory of Enteric Pathogens, Mannerheimintie 166, FIN-00300 Helsinki, Finland. Phone: 358-9-47448245. Fax: 358-9-47448238. E-mail: anja.siitonen{at}ktl.fi.

Journal of Clinical Microbiology, July 1999, p. 2176-2182, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Soto, S. M., Rodriguez, I., Rodicio, M. R., Vila, J., Mendoza, M. C. (2006). Detection of virulence determinants in clinical strains of Salmonella enterica serovar Enteritidis and mapping on macrorestriction profiles.. J Med Microbiol 55: 365-373 [Abstract] [Full Text]  
  • Betancor, L., Schelotto, F., Martinez, A., Pereira, M., Algorta, G., Rodriguez, M. A., Vignoli, R., Chabalgoity, J. A. (2004). Random Amplified Polymorphic DNA and Phenotyping Analysis of Salmonella enterica Serovar Enteritidis Isolates Collected from Humans and Poultry in Uruguay from 1995 to 2002. J. Clin. Microbiol. 42: 1155-1162 [Abstract] [Full Text]  
  • Lukinmaa, S., Miettinen, M., Nakari, U.-M., Korkeala, H., Siitonen, A. (2003). Listeria monocytogenes Isolates from Invasive Infections: Variation of Sero- and Genotypes during an 11-Year Period in Finland. J. Clin. Microbiol. 41: 1694-1700 [Abstract] [Full Text]  
  • Ratiner, Y. A., Salmenlinna, S., Eklund, M., Keskimaki, M., Siitonen, A. (2003). Serology and Genetics of the Flagellar Antigen of Escherichia coli O157:H7a,7c. J. Clin. Microbiol. 41: 1033-1040 [Abstract] [Full Text]  
  • Clark, C. G., Kruk, T. M. A. C., Bryden, L., Hirvi, Y., Ahmed, R., Rodgers, F. G. (2003). Subtyping of Salmonella enterica Serotype Enteritidis Strains by Manual and Automated PstI-SphI Ribotyping. J. Clin. Microbiol. 41: 27-33 [Abstract] [Full Text]  
  • Koort, J. M. K., Lukinmaa, S., Rantala, M., Unkila, E., Siitonen, A. (2002). Technical Improvement To Prevent DNA Degradation of Enteric Pathogens in Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 40: 3497-3498 [Abstract] [Full Text]  
  • Lukinmaa, S., Takkunen, E., Siitonen, A. (2002). Molecular Epidemiology of Clostridium perfringens Related to Food-Borne Outbreaks of Disease in Finland from 1984 to 1999. Appl. Environ. Microbiol. 68: 3744-3749 [Abstract] [Full Text]  
  • Liebana, E., Guns, D., Garcia-Migura, L., Woodward, M. J., Clifton-Hadley, F. A., Davies, R. H. (2001). Molecular Typing of Salmonella Serotypes Prevalent in Animals in England: Assessment of Methodology. J. Clin. Microbiol. 39: 3609-3616 [Abstract] [Full Text]  
  • JANTUNEN, M. E., SAXEN, H., LUKINMAA, S., ALA-HOUHALA, M., SIITONEN, A. (2001). Genomic identity of pyelonephritogenic Escherichia coli isolated from blood, urine and faeces of children with urosepsis. J Med Microbiol 50: 650-652 [Abstract] [Full Text]  
  • Liebana, E., Garcia-Migura, L., Breslin, M. F., Davies, R. H., Woodward, M. J. (2001). Diversity of Strains of Salmonella enterica Serotype Enteritidis from English Poultry Farms Assessed by Multiple Genetic Fingerprinting. J. Clin. Microbiol. 39: 154-161 [Abstract] [Full Text]  
  • Desai, M., Threlfall, E. J., Stanley, J. (2001). Fluorescent Amplified-Fragment Length Polymorphism Subtyping of the Salmonella enterica Serovar Enteritidis Phage Type 4 Clone Complex. J. Clin. Microbiol. 39: 201-206 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»