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Journal of Clinical Microbiology, July 1999, p. 2197-2200, Vol. 37, No. 7
Unidad de Bacteriología
Experimental-Centro de Investigación1 and
Servicio de Microbiología,
Received 21 December 1998/Returned for modification 1 February
1999/Accepted 17 March 1999
The in vitro activity of fluconazole against 143 Candida spp. obtained from the bloodstreams of 143 hospitalized patients from 1995 to 1997 was studied. Susceptibility
tests were carried out by two macrodilution methods, the M27-A
and a modified M27-A method (0.165 M, pH 7/morpholinepropanesulfonic
acid-buffered RPMI 1640 medium supplemented with 20 g of
D-dextrose per liter), and by the agar diffusion method
(with 15-µg fluconazole [Neo-Sensitab] tablets). With 2 µg of
fluconazole per ml, 96.92% of 65 C. albicans isolates,
86.2% of 58 C. parapsilosis isolates 7 of 8 C. tropicalis isolates, and 1 of 6 C. glabrata isolates
were inhibited. Only one strain of C. albicans and one
strain of C. tropicalis were resistant. The agreement
between the two macrodilution methods was greater than 90% within
±2 log2 dilutions for all strains except C. glabrata (83.3%) and C. tropicalis
(87.5%). Generally, MICs were 1 log2 dilution lower in
glucose-supplemented RPMI 1640 medium. No correlation between zone
sizes and MICs was found. All strains susceptible by the diffusion test
were susceptible by the dilution method, but the converse was not
necessarily true. Interestingly, inhibition zones were smaller for
C. albicans, for which the geometric mean MIC was 0.29 µg/ml and the mean inhibition zone diameter was 25.7 mm,
while for C. parapsilosis the geometric mean MIC was 0.96 µg/ml and the mean inhibition zone diameter was 31.52 mm. In
conclusion, the two macrodilution methods give similar results. The
modified M27-A method with 2% dextrose has the advantage of shortening
the incubation time and simplifying the endpoint determination.
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Fluconazole Susceptibilities of Bloodstream Candida
sp. Isolates as Determined by National Committee for Clinical
Laboratory Standards Method M27-A and Two Other Methods
*
Corresponding author. Mailing address: Unidad de
Bacteriología Experimental, Centro de Investigación,
Hospital Universitario La Fe, Avda. Campanar no. 21, 46009 Valencia,
Spain. Phone: 34 96 398 7367. Fax: 34 96 386 8718. E-mail:
ecanton{at}san.gva.es.
Journal of Clinical Microbiology, July 1999, p. 2197-2200, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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