This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hue, F.-X.
Right arrow Articles by de Bievre, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hue, F.-X.
Right arrow Articles by de Bievre, C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 1999, p. 2434-2438, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Specific Detection of Fusarium Species in Blood and Tissues by a PCR Technique

Francois-Xavier Hue,1,* Michel Huerre,2 Marie Ange Rouffault,1 and Claude de Bievre1

Laboratoire de Mycologie Médicale1 and Laboratoire d'Histopathologie,2 Institut Pasteur, 75724 Paris cedex 15, France

Received 14 October 1998/Returned for modification 1 February 1999/Accepted 30 April 1999

Fusarium species are opportunistic nosocomial pathogens that often cause fatal invasive mycoses. We designed a primer pair that amplifies by PCR a fragment of a gene coding for the rRNA of Fusarium species. The DNAs of the main Fusarium species and Neocosmospora vasinfecta but not the DNAs from 11 medically important fungi were amplified by these primers. The lower limit of detection of the PCR system was 10 fg of Fusarium solani DNA by ethidium bromide staining. To test the ability of this PCR system to detect Fusarium DNA in tissues, we developed a mouse model of disseminated fusariosis. Using the PCR, we detected Fusarium DNA in mouse tissues and in spiked human blood. Furthermore, F. solani, Fusarium moniliforme, and Fusarium oxysporum were testing by random amplified polymorphic DNA (RAPD) analysis. The bands produced by RAPD analysis were purified, cloned, and sequenced. The information was used to design primer pairs that selectively amplified one or several Fusarium species. The method developed may be useful for the rapid detection and identification of Fusarium species both from culture and from clinical samples.

* Corresponding author. Present address: Institut Pasteur, Morne Jolivière, BP 484, 97165 Pointe à Pitre cedex, Guadeloupe, France. Phone: 590.896940. Fax: 590896941. E-mail: cneyret{at}ipagua.gp.

Journal of Clinical Microbiology, August 1999, p. 2434-2438, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Oechsler, R. A., Feilmeier, M. R., Ledee, D. R., Miller, D., Diaz, M. R., Fini, M. E., Fell, J. W., Alfonso, E. C. (2009). Utility of Molecular Sequence Analysis of the ITS rRNA Region for Identification of Fusarium spp. from Ocular Sources. IOVS 50: 2230-2236 [Abstract] [Full Text]  
  • Khan, Z. U., Ahmad, S., Theyyathel, A. M. (2008). Diagnostic value of DNA and (1->3)- -D-glucan detection in serum and bronchoalveolar lavage of mice experimentally infected with Fusarium oxysporum. J Med Microbiol 57: 36-42 [Abstract] [Full Text]  
  • Nucci, M., Anaissie, E. (2007). Fusarium Infections in Immunocompromised Patients. Clin. Microbiol. Rev. 20: 695-704 [Abstract] [Full Text]  
  • Cocuroccia, B., Gaido, J., Gubinelli, E., Annessi, G., Girolomoni, G. (2003). Localized Cutaneous Hyalohyphomycosis Caused by a Fusarium Species Infection in a Renal Transplant Patient. J. Clin. Microbiol. 41: 905-907 [Abstract] [Full Text]  
  • Summerbell, R. C., Schroers, H.-J. (2002). Analysis of Phylogenetic Relationship of Cylindrocarpon lichenicola and Acremonium falciforme to the Fusarium solani Species Complex and a Review of Similarities in the Spectrum of Opportunistic Infections Caused by These Fungi. J. Clin. Microbiol. 40: 2866-2875 [Abstract] [Full Text]  
  • Austen, B, McCarthy, H, Wilkins, B, Smith, A, Duncombe, A (2001). Fatal disseminated fusarium infection in acute lymphoblastic leukaemia in complete remission. J. Clin. Pathol. 54: 488-490 [Abstract] [Full Text]  
  • Tierens, K. F.M.-J., Thomma, B. P.H.J., Brouwer, M., Schmidt, J., Kistner, K., Porzel, A., Mauch-Mani, B., Cammue, B. P.A., Broekaert, W. F. (2001). Study of the Role of Antimicrobial Glucosinolate-Derived Isothiocyanates in Resistance of Arabidopsis to Microbial Pathogens. Plant Physiol. 125: 1688-1699 [Abstract] [Full Text]  
  • Jaeger, E. E. M., Carroll, N. M., Choudhury, S., Dunlop, A. A. S., Towler, H. M. A., Matheson, M. M., Adamson, P., Okhravi, N., Lightman, S. (2000). Rapid Detection and Identification of Candida, Aspergillus, and Fusarium Species in Ocular Samples Using Nested PCR. J. Clin. Microbiol. 38: 2902-2908 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»