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Journal of Clinical Microbiology, August 1999, p. 2694-2696, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Versatile Fluorescent Staining of Fungi in Clinical Specimens by Using the Optical Brightener Blankophor

R. Rüchel* and Meike Schaffrinski

Department of Medical Microbiology, University of Göttingen, Göttingen, Germany

Received 3 November 1998/Returned for modification 12 December 1998/Accepted 9 April 1999

Fluorescent staining of fungi in clinical specimens with the optical brightener Blankophor can be performed concomitantly with maceration of surrounding tissue and may be accelerated by heating. The procedure is suitable for disclosing fungi in gram-stained microscopical mounts and can be used for screening of tissue sections prior to immunofluorescence.

* Corresponding author. Mailing address: Dept. of Medical Microbiology, Institute of Hygiene, University of Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany. Phone: 49-551-395-855. Fax: 49-551-395-860. E-mail: rrueche{at}gwdg.de.

Journal of Clinical Microbiology, August 1999, p. 2694-2696, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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