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Journal of Clinical Microbiology, August 1999, p. 2706-2708, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Human Rotavirus Strains with the P[14] Genotype by PCR

Serenella Arista,1,* Esmeralda Vizzi,1 Cristina Alaimo,1 Daniela Palermo,2 and Antonio Cascio2

Dipartimento di Igiene e Microbiologia, Università di Palermo,1 and Istituto di Patologia Infettiva e Virologia, Università di Palermo c/o Ospedale dei Bambini "G. Di Cristina,"2 Palermo, Italy

Received 6 October 1998/Returned for modification 25 March 1999/Accepted 10 May 1999

A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].

* Corresponding author. Mailing address: Dipartimento di Igiene e Microbiologia, Università di Palermo, Via del Vespro n. 133, I-90127 Palermo, Italy. Phone: 39 091 6553667. Fax: 39 091 6553674. E-mail: cascioa{at}unipa.it.

Journal of Clinical Microbiology, August 1999, p. 2706-2708, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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