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Journal of Clinical Microbiology, August 1999, p. 2715-2716, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Multidrug-Resistant Tuberculosis Microepidemic Caused by Genetically Closely Related Mycobacterium tuberculosis Strains

M. Kubín,1,* M. Havelková,1 I. Hyncicová,1 Z. Svecová,2 J. Kaustová,3 K. Kremer,4 and D. van Soolingen4

National Institute of Public Health, Prague,1 Mycobacteriology Unit, Janov-Mirosov, Department of Clinical Microbiology, Faculty of Medicine, Plzen,2 and Regional Institute of Hygiene, Ostrava,3 Czech Republic, and National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands4

Received 19 August 1998/Returned for modification 26 February 1999/Accepted 21 May 1999

IS6110 DNA fingerprinting was used to characterize an outbreak of multidrug-resistant tuberculosis in 21 individuals (17 males and 4 females) living in or roaming among four distantly separated areas in the Czech Republic. The restriction fragment length polymorphism (RFLP) analysis separated the collected Mycobacterium tuberculosis strains into group A, including 14 patients with six IS6110 copies, and group B, with 7 patients displaying highly similar RFLP patterns but with two additional IS6110 bands. A switch from pattern A to pattern B was observed in one patient, and the subsequent detection of subclone B in seven more individuals has been explained by the instability of DNA genotypes caused by transposition of IS6110 elements.

* Corresponding author. Mailing address: National Institute of Public Health, Srobárova 48, CZ-100 42 Prague, Czech Republic. Phone: 420-2-6708-2424. Fax: 420-2-6731-1188. E-mail: kubin{at}toaster.szu.cz.

Journal of Clinical Microbiology, August 1999, p. 2715-2716, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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