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Journal of Clinical Microbiology, August 1999, p. 2719-2722, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Detection and Identification of Intimin Alleles in Pathogenic Escherichia coli by Multiplex PCR

Sean D. Reid, David J. Betting, and Thomas S. Whittam*

Institute of Molecular Evolutionary Genetics, Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802

Received 25 January 1999/Accepted 20 March 1999

A multiplex PCR was designed to detect the eae gene and simultaneously identify specific alleles in pathogenic Escherichia coli. The method was tested on 87 strains representing the diarrheagenic E. coli clones. The results show that the PCR assay accurately detects eae and resolves alleles encoding the alpha , beta , and gamma  intimin variants.

* Corresponding author. Mailing address: IMEG, Dept. of Biology, 208 Mueller Laboratory, Pennsylvania State University, University Park, PA 16802. Phone: (814) 863-1970. Fax: (814) 865-9131. E-mail: tsw1{at}psu.edu.

Journal of Clinical Microbiology, August 1999, p. 2719-2722, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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