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Journal of Clinical Microbiology, September 1999, p. 2772-2776, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

PCR-Based Methods for Genotyping Viridans Group Streptococci

Sharmin Alam,1 Susan R. Brailsford,1 Robert A. Whiley,2 and David Beighton1,*

Joint Microbiology Research Unit, Guy's, King's and St. Thomas' Dental Institute, London SE5 9RW,1 and Department of Oral Microbiology, Queen Mary and Westfield College, Division of Dentistry, Whitechapel, E1 2AD,2 England

Received 11 February 1999/Returned for modification 8 April 1999/Accepted 7 June 1999

Standard repetitive extragenic palindromic (REP)-PCR, enterobacterial repetitive intergenic consensus-PCR, and Salmonella enteritidis repetitive element-PCR methods for bacterial strain typing were performed with DNA extracted by boiling members of each of the currently recognized species of human viridans group streptococci. Each of the methods was reproducible. The unique isolates (n = 72) from 15 species of viridans group streptococci were readily distinguishable, with no two isolates showing greater than 90% per cent similarity. The majority of strains exhibited much less than 90% similarity. Isolates identical by REP-PCR were also identical by the other two methods. These PCR-based typing methods, although they do not permit determination of the species of the isolates, are simple to perform and are suitable for clinical and ecological investigations of viridans group streptococci.

* Corresponding author. Mailing address: Joint Microbiology Research Unit, Guy's, King's, and St. Thomas' Dental Institute, Caldecot Rd., Denmark Hill, London SE5 9RW, England. Phone: 44-171-346-3272. Fax: 44-171-346-3073. E-mail: david.beighton{at}kcl.ac.uk.

Journal of Clinical Microbiology, September 1999, p. 2772-2776, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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