This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Griethuysen, A.
Right arrow Articles by Kluytmans, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Griethuysen, A.
Right arrow Articles by Kluytmans, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 1999, p. 2789-2792, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Slide Latex Agglutination Test for Detection of Methicillin Resistance in Staphylococcus aureus

Arjanne van Griethuysen,1,* Miranda Pouw,2 Nan van Leeuwen,2 Max Heck,2 Piet Willemse,3 Anton Buiting,1 and Jan Kluytmans3

Department of Clinical Microbiology, St. Elisabeth Hospital, Tilburg,1 National Institute of Public Health and Environmental Protection, Bilthoven,2 and Department of Clinical Microbiology, St. Ignatius Hospital, Breda,3 The Netherlands

Received 8 March 1999/Returned for modification 13 April 1999/Accepted 20 May 1999

The MRSA screen test (Denka Seiken Co., Ltd.), a commercially available, rapid (20-min) slide latex agglutination test for the determination of methicillin resistance by detection of PBP 2a in Staphylococcus aureus, was compared with the oxacillin agar screen test and PCR detection of the mecA gene. A total of 563 S. aureus isolates were tested. Two hundred ninety-six of the isolates were methicillin-susceptible isolates from cultures of blood from consecutive patients. Also, 267 methicillin-resistant isolates that comprised 248 different phage types were tested. Methicillin resistance was defined as the presence of the mecA gene. Of the 267 mecA gene-positive isolates, 263 were positive by the MRSA screen test (sensitivity, 98.5%), and all the mecA-gene negative strains were negative by the MRSA screen test (specificity, 100%). The oxacillin agar screen test detected methicillin resistance in 250 of the mecA gene-positive isolates (sensitivity, 93.6%). The sensitivity of the MRSA screen test was statistically significantly higher than the sensitivity of the oxacillin agar screen test (P < 0.05). The MRSA screen test is a highly sensitive and specific test for the detection of methicillin resistance. Also, it offers results within half an hour and is easy to perform, which makes this test a valuable tool in the ongoing battle against methicillin-resistant S. aureus.

* Corresponding author. Mailing address: Department of Clinical Microbiology, St. Elisabeth Hospital, P.O. Box 747, 5000 AS Tilburg, The Netherlands. Phone: (31) 13-5392650. Fax: (31) 13-5441264. E-mail: a.vgriethuysen{at}worldonline.nl.

Journal of Clinical Microbiology, September 1999, p. 2789-2792, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mencacci, A., Montecarlo, I., Gonfia, F., Moretti, A., Cardaccia, A., Farinelli, S., Pagliochini, M. R., Giuliani, A., Basileo, M., Pasticci, M. B., Bistoni, F. (2009). Comparison of the BD Phoenix System with the Cefoxitin Disk Diffusion Test for Detection of Methicillin Resistance in Staphylococcus aureus and Coagulase-Negative Staphylococci. J. Clin. Microbiol. 47: 2288-2291 [Abstract] [Full Text]  
  • Diederen, B. M. W., van Duijn, I., Willemse, P., Kluytmans, J. A. J. W. (2006). In Vitro Activity of Daptomycin against Methicillin-Resistant Staphylococcus aureus, Including Heterogeneously Glycopeptide-Resistant Strains.. Antimicrob. Agents Chemother. 50: 3189-3191 [Abstract] [Full Text]  
  • Diederen, B. M. W., van Leest, M.-L., van Duijn, I., Willemse, P., van Keulen, P. H. J., Kluytmans, J. A. J. W. (2006). Performance of MRSA ID, a New Chromogenic Medium for Detection of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 44: 586-588 [Abstract] [Full Text]  
  • Brown, D. F. J., Edwards, D. I., Hawkey, P. M., Morrison, D., Ridgway, G. L., Towner, K. J., Wren, M. W. D., on behalf of the Joint Working Party of the Britis, (2005). Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA). J Antimicrob Chemother 56: 1000-1018 [Abstract] [Full Text]  
  • Bressler, A. M., Williams, T., Culler, E. E., Zhu, W., Lonsway, D., Patel, J. B., Nolte, F. S. (2005). Correlation of Penicillin Binding Protein 2a Detection with Oxacillin Resistance in Staphylococcus aureus and Discovery of a Novel Penicillin Binding Protein 2a Mutation. J. Clin. Microbiol. 43: 4541-4544 [Abstract] [Full Text]  
  • Kaplan, S., Marlowe, E. M., Hogan, J. J., Doymaz, M., Bruckner, D. A., Simor, A. E. (2005). Sensitivity and Specificity of a Rapid rRNA Gene Probe Assay for Simultaneous Identification of Staphylococcus aureus and Detection of mecA. J. Clin. Microbiol. 43: 3438-3442 [Abstract] [Full Text]  
  • Miller, M. B., Meyer, H., Rogers, E., Gilligan, P. H. (2005). Comparison of Conventional Susceptibility Testing, Penicillin-Binding Protein 2a Latex Agglutination Testing, and mecA Real-Time PCR for Detection of Oxacillin Resistance in Staphylococcus aureus and Coagulase-Negative Staphylococcus. J. Clin. Microbiol. 43: 3450-3452 [Abstract] [Full Text]  
  • Diederen, B., van Duijn, I., van Belkum, A., Willemse, P., van Keulen, P., Kluytmans, J. (2005). Performance of CHROMagar MRSA Medium for Detection of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 1925-1927 [Abstract] [Full Text]  
  • van Griethuysen, A., van Loo, I., van Belkum, A., Vandenbroucke-Grauls, C., Wannet, W., van Keulen, P., Kluytmans, J. (2005). Loss of the mecA Gene during Storage of Methicillin-Resistant Staphylococcus aureus Strains. J. Clin. Microbiol. 43: 1361-1365 [Abstract] [Full Text]  
  • Warren, D. K., Liao, R. S., Merz, L. R., Eveland, M., Dunne, W. M. Jr. (2004). Detection of Methicillin-Resistant Staphylococcus aureus Directly from Nasal Swab Specimens by a Real-Time PCR Assay. J. Clin. Microbiol. 42: 5578-5581 [Abstract] [Full Text]  
  • Pottumarthy, S., Schapiro, J. M., Prentice, J. L., Houze, Y. B., Swanzy, S. R., Fang, F. C., Cookson, B. T. (2004). Clinical Isolates of Staphylococcus intermedius Masquerading as Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 42: 5881-5884 [Abstract] [Full Text]  
  • Chapin, K. C., Musgnug, M. C. (2004). Evaluation of Penicillin Binding Protein 2a Latex Agglutination Assay for Identification of Methicillin-Resistant Staphylococcus aureus Directly from Blood Cultures. J. Clin. Microbiol. 42: 1283-1284 [Abstract] [Full Text]  
  • Van Griethuysen, A., Van 't Veen, A., Buiting, A., Walsh, T., Kluytmans, J. (2003). High Percentage of Methicillin-Resistant Staphylococcus aureus Isolates with Reduced Susceptibility to Glycopeptides in The Netherlands. J. Clin. Microbiol. 41: 2487-2491 [Abstract] [Full Text]  
  • Krishnan, P U, Miles, K, Shetty, N (2002). Detection of methicillin and mupirocin resistance in Staphylococcus aureus isolates using conventional and molecular methods: a descriptive study from a burns unit with high prevalence of MRSA. J. Clin. Pathol. 55: 745-748 [Abstract] [Full Text]  
  • Felten, A., Grandry, B., Lagrange, P. H., Casin, I. (2002). Evaluation of Three Techniques for Detection of Low-Level Methicillin-Resistant Staphylococcus aureus (MRSA): a Disk Diffusion Method with Cefoxitin and Moxalactam, the Vitek 2 System, and the MRSA-Screen Latex Agglutination Test. J. Clin. Microbiol. 40: 2766-2771 [Abstract] [Full Text]  
  • Kluytmans, J., Van Griethuysen, A., Willemse, P., Van Keulen, P. (2002). Performance of CHROMagar Selective Medium and Oxacillin Resistance Screening Agar Base for Identifying Staphylococcus aureus and Detecting Methicillin Resistance. J. Clin. Microbiol. 40: 2480-2482 [Abstract] [Full Text]  
  • Sakoulas, G., Eliopoulos, G. M., Moellering, R. C. Jr., Wennersten, C., Venkataraman, L., Novick, R. P., Gold, H. S. (2002). Accessory Gene Regulator (agr) Locus in Geographically Diverse Staphylococcus aureus Isolates with Reduced Susceptibility to Vancomycin. Antimicrob. Agents Chemother. 46: 1492-1502 [Abstract] [Full Text]  
  • Jonas, D., Speck, M., Daschner, F. D., Grundmann, H. (2002). Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs. J. Clin. Microbiol. 40: 1821-1823 [Abstract] [Full Text]  
  • Sakoulas, G., Gold, H. S., Venkataraman, L., DeGirolami, P. C., Eliopoulos, G. M., Qian, Q. (2001). Methicillin-Resistant Staphylococcus aureus: Comparison of Susceptibility Testing Methods and Analysis of mecA-Positive Susceptible Strains. J. Clin. Microbiol. 39: 3946-3951 [Abstract] [Full Text]  
  • Louie, L., Majury, A., Goodfellow, J., Louie, M., Simor, A. E. (2001). Evaluation of a Latex Agglutination Test (MRSA-Screen) for Detection of Oxacillin Resistance in Coagulase-Negative Staphylococci. J. Clin. Microbiol. 39: 4149-4151 [Abstract] [Full Text]  
  • Yamazumi, T., Furuta, I., Diekema, D. J., Pfaller, M. A., Jones, R. N. (2001). Comparison of the Vitek Gram-Positive Susceptibility 106 Card, the MRSA-Screen Latex Agglutination Test, and mecA Analysis for Detecting Oxacillin Resistance in a Geographically Diverse Collection of Clinical Isolates of Coagulase-Negative Staphylococci. J. Clin. Microbiol. 39: 3633-3636 [Abstract] [Full Text]  
  • Horstkotte, M. A., Knobloch, J. K.-M., Rohde, H., Mack, D. (2001). Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci by a Penicillin-Binding Protein 2a-Specific Latex Agglutination Test. J. Clin. Microbiol. 39: 3700-3702 [Abstract] [Full Text]  
  • Swenson, J. M., Williams, P. P., Killgore, G., O'Hara, C. M., Tenover, F. C. (2001). Performance of Eight Methods, Including Two New Rapid Methods, for Detection of Oxacillin Resistance in a Challenge Set of Staphylococcus aureus Organisms. J. Clin. Microbiol. 39: 3785-3788 [Abstract] [Full Text]  
  • Brown, D. F. J., Walpole, E. (2001). Evaluation of the Mastalex latex agglutination test for methicillin resistance in Staphylococcus aureus grown on different screening media. J Antimicrob Chemother 47: 187-189 [Abstract] [Full Text]  
  • Yamazumi, T., Marshall, S. A., Wilke, W. W., Diekema, D. J., Pfaller, M. A., Jones, R. N. (2001). Comparison of the Vitek Gram-Positive Susceptibility 106 Card and the MRSA-Screen Latex Agglutination Test for Determining Oxacillin Resistance in Clinical Bloodstream Isolates of Staphylococcus aureus. J. Clin. Microbiol. 39: 53-56 [Abstract] [Full Text]  
  • van Griethuysen, A., Bes, M., Etienne, J., Zbinden, R., Kluytmans, J. (2001). International Multicenter Evaluation of Latex Agglutination Tests for Identification of Staphylococcus aureus. J. Clin. Microbiol. 39: 86-89 [Abstract] [Full Text]  
  • Hussain, Z., Stoakes, L., Garrow, S., Longo, S., Fitzgerald, V., Lannigan, R. (2000). Rapid Detection of mecA-Positive and mecA-Negative Coagulase-Negative Staphylococci by an Anti-Penicillin Binding Protein 2a Slide Latex Agglutination Test. J. Clin. Microbiol. 38: 2051-2054 [Abstract] [Full Text]  
  • Louie, L., Matsumura, S. O., Choi, E., Louie, M., Simor, A. E. (2000). Evaluation of Three Rapid Methods for Detection of Methicillin Resistance in Staphylococcus aureus. J. Clin. Microbiol. 38: 2170-2173 [Abstract] [Full Text]  
  • Merlino, J., Leroi, M., Bradbury, R., Veal, D., Harbour, C. (2000). New Chromogenic Identification and Detection of Staphylococcus aureus and Methicillin-Resistant S. aureus. J. Clin. Microbiol. 38: 2378-2380 [Abstract] [Full Text]  
  • Marriott, D. J. E., Karagiannis, T., Harkness, J. L., Kearney;, P., Cavassini, M., Wenger, A., Jaton, K., Bille, J., Blanc, D. S. (1999). Further Evaluation of the MRSA-Screen Kit for Rapid Detection of Methicillin Resistance. J. Clin. Microbiol. 37: 3783-3784 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»