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Journal of Clinical Microbiology, September 1999, p. 2910-2912, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Polymorphism of the Human Immunodeficiency Virus Type 1 (HIV-1) Protease Gene and Response of HIV-1-Infected Patients to a Protease Inhibitor

Philippe Bossi,1,2 Mireille Mouroux,1 Anne Yvon,1 François Bricaire,2 Henri Agut,1 Jean-Marie Huraux,1 Christine Katlama,2 and Vincent Calvez1,*

Departments of Virology1 and Infectious Diseases,2 Pitié-Salpêtrière Hospital, Paris, France

Received 4 January 1999/Returned for modification 19 April 1999/Accepted 14 June 1999

In order to analyze the impact of protease gene polymorphism on response to regimens containing a protease inhibitor, the entire protease coding domain from 58 human immunodeficiency virus type 1 (HIV-1)-infected patients who were protease inhibitor naive was sequenced before therapy was started. Plasma HIV-1 RNA levels were measured at baseline and at month 3 and month 6 after treatment. All patients were treated with a combination of two reverse transcriptase inhibitors and a protease inhibitor (saquinavir EOF [n = 28], ritonavir [n = 16], or indinavir [n = 14]). Before treatment, 30 different positions whose codons differed from the subtype B consensus sequence were observed. Major mutations associated with protease inhibitor resistance were not observed. No statistical correlation between the number of amino acid differences and the treatment efficacy at month 3 (-2.4 log) or month 6 (-2.7 log) was observed. At baseline, genotypic analysis of the HIV-1 protease gene of patients who have never received a protease inhibitor does not allow prediction of the efficacy of regimens containing a protease inhibitor.

* Corresponding author. Mailing address: Department of Virology---CERVI, Hôpital Pitié-Salpêtrière, 47-83 Bd de l'Hôpital, 75651 Paris Cedex 13, France. Phone: 33 1 42 17 74 09. Fax: 33 1 42 17 74 11. E-mail: vincent.calvez{at}psl.ap-hop-paris.fr.

Journal of Clinical Microbiology, September 1999, p. 2910-2912, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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