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Journal of Clinical Microbiology, September 1999, p. 3020-3021, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of Commercial Amies Transport Systems with In-House Amies Medium for Recovery of Neisseria gonorrhoeae

Donna S. Thompson* and Susanne A. French

Orillia Public Health Laboratory, Ministry of Health, Ontario, Canada L3V 6K5

Received 18 March 1999/Returned for modification 27 April 1999/Accepted 4 June 1999

Microbiologists are still encumbered by the variable performance of Amies charcoal transport medium in recovery of Neisseria gonorrhoeae. The objective of this study was to evaluate and select a good quality commercial system to replace our in-house preparation. We adsorbed 0.1 ml of a suspension from 30 gonococcal isolates onto each swab type and replaced the swab into the transport medium. We plated the swabs to New York City medium at 0, 24, 48, 72, and 96 h. We compared the survival of each isolate in the commercial Amies transport systems with that in our in-house Amies transport medium. The best recovery was observed with Copan transport systems. Some systems are inadequate and unacceptable for culture of gonococci.

* Corresponding author. Mailing address: Orillia Public Health Laboratory, 750 Memorial Ave., Orillia, Ontario, Canada L3V 6K5. Phone: 705 325 7449. Fax: 705 329 6001. E-mail: thompsond{at}health.moh.gov.on.ca.

Journal of Clinical Microbiology, September 1999, p. 3020-3021, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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