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Journal of Clinical Microbiology, January 2000, p. 133-137, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Differentiating Taenia solium and Taenia saginata Infections by Simple Hematoxylin-Eosin Staining and PCR-Restriction Enzyme Analysis

H. Mayta,1 A. Talley,2 R. H. Gilman,1,3,* J. Jimenez,1 M. Verastegui,1,4 M. Ruiz,1 H. H. Garcia,1,4,5 and A. E. Gonzalez6

Infectious Diseases Laboratory, Department of Pathology,1 and Department of Microbiology,4 Universidad Peruana Cayetano Heredia, Department of Transmissible Diseases, Instituto de Ciencias Neurológicas,5 and Department of Public Health, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos,6 Lima, Peru; Mount Sinai School of Medicine, New York, New York2; and Department of International Health, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland3

Received 5 May 1999/Returned for modification 17 May 1999/Accepted 24 August 1999

Species-specific identification of human tapeworm infections is important for public health purposes, because prompt identification of Taenia solium carriers may prevent further human cysticercosis infections (a major cause of acquired epilepsy). Two practical methods for the differentiation of cestode proglottids, (i) routine embedding, sectioning, and hematoxylin-eosin (HE) staining and (ii) PCR with restriction enzyme analysis (PCR-REA), were tested on samples from 40 individuals infected with T. solium (n = 34) or Taenia saginata (n = 6). Microscopic examination of HE staining of sections from 24 cases, in which conserved proglottids were recovered, clearly revealed differences in the number of uterine branches. Distinct restriction patterns for T. solium and T. saginata were observed when the PCR products containing the ribosomal 5.8S gene plus internal transcribed spacer regions were digested with either AluI, DdeI, or MboI. Both HE histology and PCR-REA are useful techniques for differentiating T. solium from T. saginata. Importantly, both techniques can be used in zones of endemicity. HE histology is inexpensive and is currently available in most regions of endemicity, and PCR-REA can be performed in most hospital centers already performing PCR without additional equipment or the use of radioactive material.

* Corresponding author. Mailing address: Department of International Health, Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe St., Baltimore, MD 21205. Phone: (410) 614 3959. Fax: (410) 614 5050. E-mail: rgilman{at}jhsph.edu.

Journal of Clinical Microbiology, January 2000, p. 133-137, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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