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Journal of Clinical Microbiology, January 2000, p. 431-433, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Serological Detection of Capillaria hepatica by Indirect Immunofluorescence Assay

Martina Juncker-Voss,1,* Heinrich Prosl,1 Helga Lussy,2 Ulrike Enzenberg,3 Herbert Auer,4 and Norbert Nowotny2

Institute of Parasitology and Zoology1 and Institute of Virology,2 University of Veterinary Medicine, A-1210 Vienna, Schönbrunner Tiergarten Ges.m.b.H., A-1130 Vienna,3 and Department of Medical Parasitology, Clinical Institute of Hygiene, University of Vienna, A-1095 Vienna,4 Austria

Received 25 January 1999/Returned for modification 16 March 1999/Accepted 19 October 1999

In this paper, a serological assay for the detection of antibodies to Capillaria hepatica, a zoonotic parasite, is described. In the past, the only way of detecting Capillaria hepatica was to perform a liver biopsy. The indirect immunofluorescence (IIF) assay, based on liver sections of naturally infected mice and human serum samples, is suitable for detecting early stages of human infections and for screening purposes. No cross-reactivity with other parasitic infections was detected. We have applied the IIF assay to serum samples of 60 employees of the Zoological Garden of Vienna, Schönbrunn, Austria, and found one positive and one questionable sample.


* Corresponding author. Mailing address: Institute of Parasitology and Zoology, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria. Phone: 43 1 25077 2220. Fax: 43 1 25077 2290. E-mail: Martina.Juncker{at}vu-wien.ac.at.


Journal of Clinical Microbiology, January 2000, p. 431-433, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



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