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Journal of Clinical Microbiology, October 2000, p. 3572-3576, Vol. 38, No. 10
Regional Tuberculosis Genotyping
Laboratory1 and Departments of
Medicine,2
Microbiology/Immunology,4
Pathology,5 and
Anatomy,6 University of Arkansas for
Medical Sciences, and Central Arkansas Veterans Healthcare
System, Arkansas Department of Health,3 Little
Rock, Arkansas
Received 22 March 2000/Returned for modification 10 July
2000/Accepted 18 July 2000
Several genetic loci have been utilized to genotype isolates of
Mycobacterium tuberculosis. A shortcoming of the most
commonly used method, IS6110 fingerprinting, is that it
does not adequately discriminate between isolates having few copies of
IS6110. This study was undertaken to compare pTBN12
fingerprinting of polymorphic GC-rich repetitive sequence genes and
spoligotyping of the direct repeat locus as secondary typing procedures
for M. tuberculosis isolates having fewer than six copies
of IS6110. A total of 88 isolates (100% of the isolates
with fewer than six copies of IS6110 isolated in Arkansas
during 1996 and 1997) were included in this study. Among the 88 isolates, 34 different IS6110 patterns were observed, 10 of
which were shared by more than 1 isolate, involving a total of 64 isolates. The 64 isolates were subdivided into 13 clusters (containing
37 isolates) and 27 unique isolates based on a combination of
IS6110 and pTBN12 fingerprinting and into 11 clusters
(containing 51 isolates) and 13 unique isolates based on a combination
of IS6110 fingerprinting and spoligotyping. Identical spoligotypes were found among isolates having different
IS6110 patterns, as well as among isolates showing
different pTBN12 patterns. In contrast, all isolates that had different
IS6110 patterns were found to be unique by pTBN12 typing.
The clustering rate was 73, 58, and 42%, respectively, for
IS6110 fingerprinting alone, IS6110 fingerprinting and spoligotyping combined, and IS6110 and
pTBN12 combined fingerprinting. The data indicate that the pTBN12
method has greater discriminating power among low-copy-number isolates than does spoligotyping.
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Spoligotyping and Polymorphic GC-Rich Repetitive
Sequence Fingerprinting of Mycobacterium tuberculosis
Strains Having Few Copies of IS6110
*
Corresponding author. Mailing address: Central Arkansas
Veterans Healthcare System, Medical Research Service, LR/151, 4300 West
7th St., Little Rock, AR 72205. Phone: (501) 257-4829. Fax: (501)
664-6748. E-mail: cavedonald{at}exchange.uams.edu.
Journal of Clinical Microbiology, October 2000, p. 3572-3576, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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