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Journal of Clinical Microbiology, October 2000, p. 3822-3824, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection and Quantitation of Human Immunodeficiency Virus Type 1 in the Female Genital Tract

Penny Baron,1 James Bremer,2 Steven S. Wasserman,3 Marek Nowicki,4 Barbara Driscoll,5 Bruce Polsky,1 Andrea Kovacs,4 and Patricia S. Reichelderfer6,*,dagger For The Division Of Aids Treatment Research Initiative 009 Study Group

St. Luke's Hospital, New York, New York 100251; Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 606122; University of Maryland School of Medicine, Baltimore, Maryland 212013; University of Southern California, Los Angeles, California 900334; State University of New York Health Science Center at Brooklyn, Brooklyn, New York 11203-20985; and National Institute of Child Health and Human Development, Bethesda, Maryland 208926

Received 22 February 2000/Returned for modification 20 April 2000/Accepted 12 June 2000

Human immunodeficiency virus type 1 (HIV-1) was detected in the genital tracts of 59% of 225 women by RNA PCR and in 7% of the women by culture. In a comparison of two sampling methods, endocervical swabs were more sensitive than cervicovaginal lavage for HIV-1 RNA detection by PCR but not by culture and their sensitivity was independent of the concentration of HIV-1 RNA.

* Corresponding author. Mailing address: National Institute of Child Health and Human Development, National Institutes of Health, 6100 Executive Blvd., Room 8B13D, Bethesda, MD 20852. Phone: (301) 435-6991. Fax: (301) 480-1972. E-mail: pr20f{at}nih.gov.

dagger This study group also includes D. Burns, M. Cohen, S. Beckner, J. Cohn, P. Miotti, R. Greenblatt, S. Wahl, M. Redford, L. Corey, M. Young, K. Weber, A. Landay, and B. Weiser.

Journal of Clinical Microbiology, October 2000, p. 3822-3824, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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