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Journal of Clinical Microbiology, November 2000, p. 4076-4079, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Diagnostic Performance of the Roche AMPLICOR PCR in Detecting Neisseria gonorrhoeae in Genitourinary Specimens from Female Sex Workers in Cotonou, Benin

L. Mukenge-Tshibaka,1 M. Alary,1,* F. Bernier,2,dagger E. van Dyck,3 C. M. Lowndes,1,Dagger A. Guédou,4 S. Anagonou,4,5 and J. R. Joly2,§

Epidemiology Research Group, Hôpital du Saint-Sacrement du CHA and Université Laval, Québec,1 and Department of Microbiology and Immunology, Université de Montréal, Montreal,2 Canada; Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium3; and Microbiology Laboratory, Centre National Hospitalier Universitaire,4 and Programme National de Lutte Contre le SIDA et les MST,5 Cotonou, Benin

Received 1 March 2000/Returned for modification 17 May 2000/Accepted 16 August 2000

The objective of this study was to evaluate the diagnostic performance of the Roche multiplex AMPLICOR Chlamydia trachomatis/Neisseria gonorrhoeae PCR test for the detection of Neisseria gonorrhoeae infection in female urine specimens and wet and dry endocervical swabs. Endocervical swabs and urine specimens were collected from 342 female sex workers from Cotonou, Benin, and were tested using the AMPLICOR C. trachomatis/N. gonorrhoeae test (Roche Diagnostic Systems, Inc., Branchburg, N.J.) with internal control detection. Endocervical swabs were also cultured on Thayer-Martin medium. A series of alternate standards that included a combination of all the tests but not the test being evaluated was used to assess the performance of the test with each type of specimen. The sensitivity, specificity, and positive and negative predictive values for the urine were 53.8, 98.9, 93.5, and 87.5%, respectively. Corresponding figures for the wet swab were 91.5, 100, 100, and 97.4%, respectively. Those for the dry swab were 96.3, 96.2, 88.5, and 98.8%, respectively. Based on this study, the AMPLICOR PCR assay showed a low sensitivity for detection of N. gonorrhoeae infection in urine specimens, whereas the test was found to be highly sensitive and specific with endocervical specimens.

* Corresponding author. Mailing address: Centre de Recherche, Hôpital du Saint-Sacrement du CHA, 1050 Chemin Ste-Foy, Québec, Québec G1S 4L8, Canada. Phone: (418) 682-7387. Fax: (418) 682-7949. E-mail: michel.alary{at}gre.ulaval.ca.

dagger Present address: Héma-Québec, Montréal, Canada.

Dagger Present address: Department of Social Science and Medicine, Imperial College, London, United Kingdom.

§ Present address: Health Protection Division, Institut National de Santé Publique du Québec, Québec, Canada.

Journal of Clinical Microbiology, November 2000, p. 4076-4079, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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