This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fouchier, R. A. M.
Right arrow Articles by Osterhaus, A. D. M. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fouchier, R. A. M.
Right arrow Articles by Osterhaus, A. D. M. E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2000, p. 4096-4101, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of Influenza A Viruses from Different Species by PCR Amplification of Conserved Sequences in the Matrix Gene

Ron A. M. Fouchier,* Theo M. Bestebroer, Sander Herfst, Liane Van Der Kemp, Guus F. Rimmelzwaan, and Albert D. M. E. Osterhaus

National Influenza Center and Department of Virology, Erasmus University, Rotterdam, The Netherlands

Received 11 May 2000/Returned for modification 27 July 2000/Accepted 5 September 2000

The recently raised awareness of the threat of a new influenza pandemic has stimulated interest in the detection of influenza A viruses in human as well as animal secretions. Virus isolation alone is unsatisfactory for this purpose because of its inherent limited sensitivity and the lack of host cells that are universally permissive to all influenza A viruses. Previously described PCR methods are more sensitive but are targeted predominantly at virus strains currently circulating in humans, since the sequences of the primer sets display considerable numbers of mismatches to the sequences of animal influenza A viruses. Therefore, a new set of primers, based on highly conserved regions of the matrix gene, was designed for single-tube reverse transcription-PCR for the detection of influenza A viruses from multiple species. This PCR proved to be fully reactive with a panel of 25 genetically diverse virus isolates that were obtained from birds, humans, pigs, horses, and seals and that included all known subtypes of influenza A virus. It was not reactive with the 11 other RNA viruses tested. Comparative tests with throat swab samples from humans and fecal and cloacal swab samples from birds confirmed that the new PCR is faster and up to 100-fold more sensitive than classical virus isolation procedures.

* Corresponding author. Mailing address: Department of Virology, Erasmus University Rotterdam, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands. Phone: 31 10 4088066. Fax: 31 10 4089485. E-mail: fouchier{at}viro.fgg.eur.nl.

Journal of Clinical Microbiology, November 2000, p. 4096-4101, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pabbaraju, K., Wong, S., Wong, A. A., Appleyard, G. D., Chui, L., Pang, X.-L., Yanow, S. K., Fonseca, K., Lee, B. E., Fox, J. D., Preiksaitis, J. K. (2009). Design and Validation of Real-Time Reverse Transcription-PCR Assays for Detection of Pandemic (H1N1) 2009 Virus. J. Clin. Microbiol. 47: 3454-3460 [Abstract] [Full Text]  
  • Cutler, J., Schleihauf, E., Hatchette, T. F., Billard, B., Watson-Creed, G., Davidson, R., Li, Y., Bastien, N., Sarwal, S., the Nova Scotia Human Swine Influenza Investigatio, (2009). Investigation of the first cases of human-to-human infection with the new swine-origin influenza A (H1N1) virus in Canada. CMAJ 181: 159-163 [Abstract] [Full Text]  
  • Kabell, S., Handberg, K., Jorgensen, P. H. (2009). Inactivation of avian influenza virus H5N2 in acidified chicken pulp. Vet Rec. 164: 532-533 [Full Text]  
  • Lang, A. S., Kelly, A., Runstadler, J. A. (2008). Prevalence and diversity of avian influenza viruses in environmental reservoirs. J. Gen. Virol. 89: 509-519 [Abstract] [Full Text]  
  • Campitelli, L., Di Martino, A., Spagnolo, D., Smith, G. J. D., Di Trani, L., Facchini, M., De Marco, M. A., Foni, E., Chiapponi, C., Martin, A. M., Chen, H., Guan, Y., Delogu, M., Donatelli, I. (2008). Molecular analysis of avian H7 influenza viruses circulating in Eurasia in 1999-2005: detection of multiple reassortant virus genotypes. J. Gen. Virol. 89: 48-59 [Abstract] [Full Text]  
  • Noppornpanth, S., Smits, S. L., Lien, T. X., Poovorawan, Y., Osterhaus, A. D. M. E., Haagmans, B. L. (2007). Characterization of Hepatitis C Virus Deletion Mutants Circulating in Chronically Infected Patients. J. Virol. 81: 12496-12503 [Abstract] [Full Text]  
  • Lebarbenchon, C., Chang, C.-M., van der Werf, S., Aubin, J.-T., Kayser, Y., Ballesteros, M., Renaud, F., Thomas, F., Gauthier-Clerc, M. (2007). Influenza A Virus in Birds during Spring Migration in the Camargue, France. J Wildl Dis 43: 789-793 [Abstract] [Full Text]  
  • D'Amico, V. L., Bertellotti, M., Baker, A. J., Diaz, L. A. (2007). Exposure of Red Knots (Calidris canutus rufa) to Select Avian Pathogens; Patagonia, Argentina. J Wildl Dis 43: 794-797 [Abstract] [Full Text]  
  • Busquets Marti, N., Serrano Del pozo, E., Casals, A. A., Nunez Garrote, J. I., Majo Masferrer, N. (2006). False-Positive Results Obtained by Following a Commonly Used Reverse Transcription-PCR Protocol for Detection of Influenza A Virus.. J. Clin. Microbiol. 44: 3845-3845 [Full Text]  
  • Quinlivan, M., Dempsey, E., Ryan, F., Arkins, S., Cullinane, A. (2005). Real-Time Reverse Transcription PCR for Detection and Quantitative Analysis of Equine Influenza Virus. J. Clin. Microbiol. 43: 5055-5057 [Abstract] [Full Text]  
  • Schrenzel, M., Oaks, J. L., Rotstein, D., Maalouf, G., Snook, E., Sandfort, C., Rideout, B. (2005). Characterization of a New Species of Adenovirus in Falcons. J. Clin. Microbiol. 43: 3402-3413 [Abstract] [Full Text]  
  • Fouchier, R. A. M., Munster, V., Wallensten, A., Bestebroer, T. M., Herfst, S., Smith, D., Rimmelzwaan, G. F., Olsen, B., Osterhaus, A. D. M. E. (2005). Characterization of a Novel Influenza A Virus Hemagglutinin Subtype (H16) Obtained from Black-Headed Gulls. J. Virol. 79: 2814-2822 [Abstract] [Full Text]  
  • Hindiyeh, M., Levy, V., Azar, R., Varsano, N., Regev, L., Shalev, Y., Grossman, Z., Mendelson, E. (2005). Evaluation of a Multiplex Real-Time Reverse Transcriptase PCR Assay for Detection and Differentiation of Influenza Viruses A and B during the 2001-2002 Influenza Season in Israel. J. Clin. Microbiol. 43: 589-595 [Abstract] [Full Text]  
  • Poon, L. L. M., Leung, C. S. W., Chan, K. H., Lee, J. H. C., Yuen, K. Y., Guan, Y., Peiris, J. S. M. (2005). Detection of Human Influenza A Viruses by Loop-Mediated Isothermal Amplification. J. Clin. Microbiol. 43: 427-430 [Abstract] [Full Text]  
  • Bueving, H.J., van der Wouden, J.C., Raat, H., Bernsen, R.M.D., de Jongste, J.C., van Suijlekom-Smit, L.W.A., Osterhaus, A.D.M.E., Rimmelzwaan, G.F., Molken, M. R.-v., Thomas, S. (2004). Influenza vaccination in asthmatic children: effects on quality of life and symptoms. Eur Respir J 24: 925-931 [Abstract] [Full Text]  
  • van Bolhuis, G. H., Rijks, J. M., Dorrestein, G. M., Rudolfova, J., van Dijk, M., Kuiken, T. (2004). Obliterative Endophlebitis in Mute Swans (Cygnus olor) Caused by Trichobilharzia sp. (Digenea: Schistosomatidae) Infection. Vet Pathol 41: 658-665 [Abstract] [Full Text]  
  • Kuiken, T., van den Hoogen, B. G., van Riel, D. A.J., Laman, J. D., van Amerongen, G., Sprong, L., Fouchier, R. A.M., Osterhaus, A. D.M.E. (2004). Experimental Human Metapneumovirus Infection of Cynomolgus Macaques (Macaca fascicularis) Results in Virus Replication in Ciliated Epithelial Cells and Pneumocytes with Associated Lesions throughout the Respiratory Tract. Am. J. Pathol. 164: 1893-1900 [Abstract] [Full Text]  
  • Trampuz, A., Prabhu, R. M., Smith, T. F., Baddour, L. M. (2004). Avian Influenza: A New Pandemic Threat?. Mayo Clin Proc. 79: 523-530 [Abstract]  
  • Maertzdorf, J., Wang, C. K., Brown, J. B., Quinto, J. D., Chu, M., de Graaf, M., van den Hoogen, B. G., Spaete, R., Osterhaus, A. D. M. E., Fouchier, R. A. M. (2004). Real-Time Reverse Transcriptase PCR Assay for Detection of Human Metapneumoviruses from All Known Genetic Lineages. J. Clin. Microbiol. 42: 981-986 [Abstract] [Full Text]  
  • Fouchier, R. A. M., Schneeberger, P. M., Rozendaal, F. W., Broekman, J. M., Kemink, S. A. G., Munster, V., Kuiken, T., Rimmelzwaan, G. F., Schutten, M., van Doornum, G. J. J., Koch, G., Bosman, A., Koopmans, M., Osterhaus, A. D. M. E. (2004). Avian influenza A virus (H7N7) associated with human conjunctivitis and a fatal case of acute respiratory distress syndrome. Proc. Natl. Acad. Sci. USA 101: 1356-1361 [Abstract] [Full Text]  
  • Quinlivan, M., Cullinane, A., Nelly, M., van Maanen, K., Heldens, J., Arkins, S. (2004). Comparison of Sensitivities of Virus Isolation, Antigen Detection, and Nucleic Acid Amplification for Detection of Equine Influenza Virus. J. Clin. Microbiol. 42: 759-763 [Abstract] [Full Text]  
  • Boivin, G., Cote, S., Dery, P., De Serres, G., Bergeron, M. G. (2004). Multiplex Real-Time PCR Assay for Detection of Influenza and Human Respiratory Syncytial Viruses. J. Clin. Microbiol. 42: 45-51 [Abstract] [Full Text]  
  • Ruest, A., Michaud, S., Deslandes, S., Frost, E. H. (2003). Comparison of the Directigen Flu A+B Test, the QuickVue Influenza Test, and Clinical Case Definition to Viral Culture and Reverse Transcription-PCR for Rapid Diagnosis of Influenza Virus Infection. J. Clin. Microbiol. 41: 3487-3493 [Abstract] [Full Text]  
  • Martina, B. E. E., Harder, T. C., Osterhaus, A. D. M. E. (2003). Genetic characterization of the unique short segment of Phocid herpesvirus type 1 reveals close relationships among alphaherpesviruses of hosts of the order Carnivora. J. Gen. Virol. 84: 1427-1430 [Abstract] [Full Text]  
  • Ellis, J. S., Zambon, M. C. (2001). Combined PCR-Heteroduplex Mobility Assay for Detection and Differentiation of Influenza A Viruses from Different Animal Species. J. Clin. Microbiol. 39: 4097-4102 [Abstract] [Full Text]  
  • Rimmelzwaan, G. F., Kuiken, T., van Amerongen, G., Bestebroer, T. M., Fouchier, R. A. M., Osterhaus, A. D. M. E. (2001). Pathogenesis of Influenza A (H5N1) Virus Infection in a Primate Model. J. Virol. 75: 6687-6691 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»