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Journal of Clinical Microbiology, November 2000, p. 4201-4207, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Application of tRNA Intergenic Spacer PCR for Identification of Enterococcus Species

Margo Baele,1,* Paul Baele,1 Mario Vaneechoutte,2 Virginie Storms,3 Patrick Butaye,1 Luc A. Devriese,1 Gerda Verschraegen,2 Monique Gillis,3 and Freddy Haesebrouck1

Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, University of Ghent, B-9820 Merelbeke,1 Department of Clinical Chemistry, Microbiology and Immunology, Faculty of Medicine, University of Ghent,2 and Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent,3 B-9000 Ghent, Belgium

Received 13 June 2000/Returned for modification 25 July 2000/Accepted 31 August 2000

tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR enabled us to discriminate for all species tested. Enterococcus faecium showed minor but reproducible differences with Enterococcus durans, while Enterococcus hirae was easily distinguishable. Enterococcus avium, Enterococcus malodoratus, and Enterococcus raffinosus generated highly similar though distinctive patterns.

* Corresponding author. Mailing address: Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133, B-9820 Merelbeke, Belgium. Phone: 32 9 264 74 34. Fax: 32 9 264 74 94. E-mail: Margo.Baele{at}rug.ac.be.

Journal of Clinical Microbiology, November 2000, p. 4201-4207, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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