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Journal of Clinical Microbiology, November 2000, p. 4260-4261, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of a Commercial DNA Enzyme Immunoassay for Detection of Enterovirus Reverse Transcription-PCR Products Amplified from Cerebrospinal Fluid Specimens

Pampee P. Young,1,2 Richard S. Buller,3 and Gregory A. Storch2,3,*

The Departments of Pathology1 and Medicine2 and The Edward Mallinckrodt Department of Pediatrics,3 Washington University School of Medicine at St. Louis Children's Hospital, St. Louis, Missouri

Received 15 June 2000/Returned for modification 20 July 2000/Accepted 23 August 2000

We evaluated the DiaSorin DNA enzyme immunoassay (DEIA) kit for detection of enteroviral reverse transcription-PCR (RT-PCR) products amplified from cerebrospinal fluid. By use of an optical density of 0.05 as the absorbance cutoff, 35% of 198 specimens were PCR positive, whereas 16% were culture positive. DEIA was rapid and sensitive and can help implement enterovirus RT-PCR in clinical laboratories.

* Corresponding author. Mailing address: Department of Pediatrics, Washington University School of Medicine at St. Louis Children's Hospital, One Children's Place, St. Louis, MO 63110. Phone: (314) 454-6079. Fax: (314) 454-2274. E-mail: storch{at}kids.wustl.edu.

Journal of Clinical Microbiology, November 2000, p. 4260-4261, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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