This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Viscidi, R. P.
Right arrow Articles by Zenilman, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Viscidi, R. P.
Right arrow Articles by Zenilman, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2000, p. 4430-4438, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of Sequencing of the por Gene and Typing of the opa Gene for Discrimination of Neisseria gonorrhoeae Strains from Sexual Contacts

Raphael P. Viscidi,1,* James C. Demma,1 Jing Gu,1 and Jonathan Zenilman2

Stanley Division of Developmental Neurovirology, Department of Pediatrics,1 and Division of Infectious Diseases, Department of Medicine,2 Johns Hopkins University School of Medicine, Baltimore, Maryland 21287

Received 27 June 2000/Returned for modification 3 September 2000/Accepted 22 September 2000

Typing of gonococcal strains is a valuable tool for the biological confirmation of sexual contacts. We have developed a typing method based on DNA sequencing of two overlapping por gene fragments generated by a heminested PCR. We compared sequencing of the por gene (POR sequencing) and typing of the opa gene (OPA typing) for the characterization of strains from 17 sexual partnerships. Both methods were highly discriminatory. A different genotype was detected in 15 of the 17 epidemiologically unconnected couples by POR sequencing and in 16 of the 17 couples by OPA typing with restriction enzyme HpaII. Within partnerships, identical genotypes were obtained from 16 of the 17 known sex contacts by POR sequencing and from 15 of the 17 by OPA typing. Compared to OPA typing, which relies on interpretation of bands in a gel, DNA sequence data offer the advantage of being objective and portable. As costs for sequencing decline, the method should become affordable for most laboratory personnel who wish to type gonococcal strains.

* Corresponding author. Mailing address: The Johns Hopkins Hospital, Blalock Building, Room 1105, 600 North Wolfe St., Baltimore, MD 21287. Phone: (410) 614-1494. Fax: (410) 955-3723. E-mail: rviscidi{at}mail.jhmi.edu.

Journal of Clinical Microbiology, December 2000, p. 4430-4438, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Liao, M., Helgeson, S., Gu, W.-M., Yang, Y., Jolly, A. M., Dillon, J.-A. R. (2009). Comparison of Neisseria gonorrhoeae Multiantigen Sequence Typing and porB Sequence Analysis for Identification of Clusters of N. gonorrhoeae Isolates. J. Clin. Microbiol. 47: 489-491 [Abstract] [Full Text]  
  • Ilina, E. N., Borovskaya, A. D., Malakhova, M. M., Vereshchagin, V. A., Kubanova, A. A., Kruglov, A. N., Svistunova, T. S., Gazarian, A. O., Maier, T., Kostrzewa, M., Govorun, V. M. (2009). Direct Bacterial Profiling by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry for Identification of Pathogenic Neisseria. J. Mol. Diagn. 11: 75-86 [Abstract] [Full Text]  
  • Ilina, E. N., Vereshchagin, V. A., Borovskaya, A. D., Malakhova, M. V., Sidorenko, S. V., Al-Khafaji, N. C., Kubanova, A. A., Govorun, V. M. (2008). Relation between Genetic Markers of Drug Resistance and Susceptibility Profile of Clinical Neisseria gonorrhoeae Strains. Antimicrob. Agents Chemother. 52: 2175-2182 [Abstract] [Full Text]  
  • Liao, M., Bell, K., Gu, W.-M., Yang, Y., Eng, N. F., Fu, W., Wu, L., Zhang, C.-G., Chen, Y., Jolly, A. M., Dillon, J.-A. R. (2008). Clusters of circulating Neisseria gonorrhoeae strains and association with antimicrobial resistance in Shanghai. J Antimicrob Chemother 61: 478-487 [Abstract] [Full Text]  
  • Bilek, N., Martin, I. M., Bell, G., Kinghorn, G. R., Ison, C. A., Spratt, B. G. (2007). Concordance between Neisseria gonorrhoeae Genotypes Recovered from Known Sexual Contacts. J. Clin. Microbiol. 45: 3564-3567 [Abstract] [Full Text]  
  • Bash, M. C., Zhu, P., Gulati, S., McKnew, D., Rice, P. A., Lynn, F. (2005). por Variable-Region Typing by DNA Probe Hybridization Is Broadly Applicable to Epidemiologic Studies of Neisseria gonorrhoeae. J. Clin. Microbiol. 43: 1522-1530 [Abstract] [Full Text]  
  • Unemo, M., Olcen, P., Jonasson, J., Fredlund, H. (2004). Molecular Typing of Neisseria gonorrhoeae Isolates by Pyrosequencing of Highly Polymorphic Segments of the porB Gene. J. Clin. Microbiol. 42: 2926-2934 [Abstract] [Full Text]  
  • Vereshchagin, V. A., Ilina, E. N., Malakhova, M. V., Zubkov, M. M., Sidorenko, S. V., Kubanova, A. A., Govorun, V. M. (2004). Fluoroquinolone-resistant Neisseria gonorrhoeae isolates from Russia: molecular mechanisms implicated. J Antimicrob Chemother 53: 653-656 [Abstract] [Full Text]  
  • Martin, I. M. C., Ghani, A., Bell, G., Kinghorn, G., Ison, C. A. (2003). Persistence of Two Genotypes of Neisseria gonorrhoeae during Transmission. J. Clin. Microbiol. 41: 5609-5614 [Abstract] [Full Text]  
  • Unemo, M., Olcen, P., Albert, J., Fredlund, H. (2003). Comparison of Serologic and Genetic porB-Based Typing of Neisseria gonorrhoeae: Consequences for Future Characterization. J. Clin. Microbiol. 41: 4141-4147 [Abstract] [Full Text]  
  • Viscidi, R. P., Demma, J. C. (2003). Genetic Diversity of Neisseria gonorrhoeae Housekeeping Genes. J. Clin. Microbiol. 41: 197-204 [Abstract] [Full Text]  
  • Moodley, P., Martin, I. M. C., Ison, C. A., Sturm, A. W. (2002). Typing of Neisseria gonorrhoeae Reveals Rapid Reinfection in Rural South Africa. J. Clin. Microbiol. 40: 4567-4570 [Abstract] [Full Text]  
  • Unemo, M., Olcen, P., Berglund, T., Albert, J., Fredlund, H. (2002). Molecular Epidemiology of Neisseria gonorrhoeae: Sequence Analysis of the porB Gene Confirms Presence of Two Circulating Strains. J. Clin. Microbiol. 40: 3741-3749 [Abstract] [Full Text]  
  • Nallapareddy, S. R., Duh, R.-W., Singh, K. V., Murray, B. E. (2002). Molecular Typing of Selected Enterococcus faecalis Isolates: Pilot Study Using Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 40: 868-876 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»