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Journal of Clinical Microbiology, December 2000, p. 4616-4620, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Genotyping of Verocytotoxin-Producing Escherichia coli O157: Comparison of Isolates of a Prevalent Phage Type by Fluorescent Amplified-Fragment Length Polymorphism and Pulsed-Field Gel Electrophoresis Analyses

David Smith,1 Geraldine Willshaw,2 John Stanley,1 and Catherine Arnold1,*

Molecular Biology Unit, Virus Reference Division,1 and Laboratory of Enteric Pathogens,2 Central Public Health Laboratory, London NW9 5HT, United Kingdom

Received 7 June 2000/Returned for modification 29 August 2000/Accepted 13 September 2000

We applied the high-resolution genotyping technique fluorescent amplified-fragment length polymorphism (FAFLP) analysis to 71 isolates of a single phage type (PT8) of pulsed-field gel electrophoresis (PFGE)-characterized verocytotoxin-producing Escherichia coli O157. Twenty-seven similar, but not identical, groupings were defined by both FAFLP analysis and the PFGE profiles. Given the FAFLP analysis conditions described here, these two methods exhibited equivalent discriminatory powers.

* Corresponding author. Mailing address: Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 020 8200 4400. Fax: 020 8200 1569. E-mail: carnold{at}phls.nhs.uk.

Journal of Clinical Microbiology, December 2000, p. 4616-4620, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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