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Journal of Clinical Microbiology, December 2000, p. 4643-4645, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

PCR-Restriction Enzyme Analysis of a Bone Marrow Isolate from a Human Immunodeficiency Virus-Positive Patient Discloses Polyclonal Infection with Two Mycobacterium avium Strains

R. S. Oliveira,1 M. P. Sircili,1 S. Y. M. Ueki,2 M. A. S. Telles,2 B. Schnabel,1 M. R. S. Briones,1 and S. C. Leão1,*

Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo-Escola Paulista de Medicina,1 and Seção de Micobactérias, Instituto Adolfo Lutz,2 São Paulo, Brazil

Received 20 July 2000/Returned for modification 17 August 2000/Accepted 20 September 2000

Polyclonal infection by Mycobacterium avium was detected by hsp65 PCR-restriction enzyme analysis (PRA) in a bone marrow isolate from an AIDS patient. Two M. avium strains, differing in colony morphology, PRA HaeIII digestion pattern, insertion element (IS) 1245 amplification, and restriction fragment length polymorphism fingerprints with IS1245 and IS1311 probes, were isolated.

* Corresponding author. Mailing address: Universidade Federal de São Paulo-Escola Paulista de Medicina, Rua Botucatu, 862 3° andar, 04023-062 São Paulo, Brazil. Phone: (55-11) 5084-3213. Fax: (55-11) 5571-6504. E-mail: sylvia{at}ecb.epm.br.

Journal of Clinical Microbiology, December 2000, p. 4643-4645, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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