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Journal of Clinical Microbiology, December 2000, p. 4668-4671, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of Bovine Herpesvirus 4 (BoHV-4) DNA in the Cell Fraction of Milk of Dairy Cattle with History of BoHV-4 Infection

Gaetano Donofrio,* Cesidio Filippo Flammini, Franco Scatozza, and Sandro Cavirani

Istituto di Malattie Infettive Profilassi e Polizia Veterinaria, Facoltà di Medicina Veterinaria, Università di Parma, 43100 Parma, Italy

Received 12 June 2000/Returned for modification 30 August 2000/Accepted 24 September 2000

We have demonstrated, by PCR and restriction enzyme analysis of the PCR product, the presence of bovine herpesvirus 4 (BoHV-4) DNA in the cell fraction of milk from dairy cattle with a history of BoHV-4 infection. We next evaluated the infectious nature of BoHV-4 DNA in those cells. Cocultivation of a BoHV-4-sensitive cell line with BoHV-4 DNA-positive milk cell samples produced cytopathic effects. The same result was obtained from frozen and thawed milk cell fraction coming from the cell milk fraction PCR-positive cows, ensuring that cells were killed and only infectious virus could be recovered after cocultivation with sensitive cells. This report shows that infectious BoHV-4 can be present in milk cells and that therefore nursing may be one of the transmission routes of BoHV-4.

* Corresponding author. Mailing address: Istituto di Malattie Infettive Profilassi e Polizia Veterinaria, Facoltà di Medicina Veterinaria, Università di Parma, 43100 Parma, Italy. Phone: 0039-0521-902669. Fax: 0039-0521-902672. E-mail: donofrio{at}unipr.it.

Journal of Clinical Microbiology, December 2000, p. 4668-4671, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bona, C., Dewals, B., Wiggers, L., Coudijzer, K., Vanderplasschen, A., Gillet, L. (2005). Short Communication: Pasteurization of Milk Abolishes Bovine Herpesvirus 4 Infectivity. J DAIRY SCI 88: 3079-3083 [Abstract] [Full Text]  
  • Donofrio, G., Cavirani, S., van Santen, V., Flammini, C. F. (2005). Potential Secondary Pathogenic Role for Bovine Herpesvirus 4. J. Clin. Microbiol. 43: 3421-3426 [Abstract] [Full Text]  
  • Gillet, L., Minner, F., Detry, B., Farnir, F., Willems, L., Lambot, M., Thiry, E., Pastoret, P.-P., Schynts, F., Vanderplasschen, A. (2004). Investigation of the Susceptibility of Human Cell Lines to Bovine Herpesvirus 4 Infection: Demonstration that Human Cells Can Support a Nonpermissive Persistent Infection Which Protects Them against Tumor Necrosis Factor Alpha-Induced Apoptosis. J. Virol. 78: 2336-2347 [Abstract] [Full Text]  


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