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Journal of Clinical Microbiology, February 2000, p. 483-488, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sequence Analysis and Clinical Significance of the iceA Gene from Helicobacter pylori Strains in Japan

Yoshiyuki Ito,1 Takeshi Azuma,1,* Shigeji Ito,1 Hiroyuki Suto,1 Hideki Miyaji,1 Yukinao Yamazaki,1 Takuji Kato,2 Yoshihiro Kohli,3 Yoshihide Keida,4 and Masaru Kuriyama1

Second Department of Internal Medicine, Fukui Medical University,1 and Fukui Prefectural University, College of Nursing,2 Fukui, Division of Internal Medicine, Aiseikai Yamashina Hospital, Kyoto,3 and Division of Internal Medicine, Okinawa Chubu Hospital, Okinawa,4 Japan

Received 7 June 1999/Returned for modification 17 August 1999/Accepted 1 November 1999

The Helicobacter pylori iceA gene was recently identified as a genetic marker for the development of peptic ulcer in a Western population. To assess the significance of iceA subtypes of H. pylori in relation to peptic ulcer, 140 Japanese clinical isolates (88 from Fukui and 52 from Okinawa) were characterized. Sequence analysis of the iceA1 gene from 25 representative Japanese strains was also carried out to identify the differences in iceA between the ulcer group and the gastritis group. The iceA1 genotype was not correlated with the presence of peptic ulceration in either area. In addition, sequence analysis led to identification of five deletions and five point mutations (a nonsense mutation or a 1-bp insertion) within the iceA1 open reading frame corresponding to previously published sequences. These mutations were identified in both clinical groups (ulcer and gastritis groups) in each area. Local DNA sequence analysis revealed that the endpoints of all five deletions coincided with direct repeats. We also found four strains that carried longer iceA1 open reading frames compared with that for strain 60190. In conclusion, carriage of an iceA1 strain does not seem to be a risk factor for peptic ulcer in Japanese subjects. The critical mutations in the iceA1 gene in some isolates from patients with peptic ulcers suggested that IceA does not participate in the pathogenesis of peptic ulcer in Japan. We also found deletion hot spots that were associated with direct repeats in iceA1 and that favored a small-deletion model of slipped mispairing events during replication. We showed that iceA1 sequence variations may be useful tools for analysis of the population genetics of H. pylori.

* Corresponding author. Mailing address: Second Department of Internal Medicine, Fukui Medical University, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan. Phone: 81-776-61-3111, ext. 2300. Fax: 81-776-61-8110. E-mail: azuma{at}fmsrsa.fukui-med.ac.jp.

Journal of Clinical Microbiology, February 2000, p. 483-488, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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