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Journal of Clinical Microbiology, February 2000, p. 526-529, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of Carbapenemase-Producing Acinetobacter baumannii in a Hospital

Ayako Takahashi,1 Sachie Yomoda,1 Isao Kobayashi,1 Toyoji Okubo,2 Mitsuko Tsunoda,2 and Shizuko Iyobe2,*

Department of Laboratory Medicine and Clinical Laboratory Center1 and Laboratory of Drug Resistance in Bacteria,2 Gunma University School of Medicine, 3-39-22, Showa-machi, Maebashi 371-8511, Japan

Received 7 September 1999/Returned for modification 22 October 1999/Accepted 15 November 1999

Acinetobacter baumannii strains resistant to both imipenem (IPM) and ceftazidime (CAZ) were isolated from 1994 through 1996 at Gunma University Hospital. Nine isolates from different inpatients were examined for carbapenem-hydrolyzing activity and for the carbapemase gene blaIMP by the PCR method. All nine isolates were carbapenemase-producing strains that hydrolyzed IPM and that harbored blaIMP. The blaIMP gene was transmissible by conjugation to an IPM-susceptible recipient strain of A. baumannii and conferred resistance to IPM, CAZ, cefotaxime (CTX), ampicillin (AMP), and piperacillin (PIP). Either intermediate or high-level resistance to amikacin (AMK) was transferred from two and five strains, respectively, concomitantly with blaIMP, and gentamicin (GEN) resistance was also transferred in one instance of high-level AMK resistance. Comparative examination of clinical isolates for resistance patterns to nine drugs, IPM, CAZ, CTX, aztreonam, AMP, PIP, AMK, GEN, and norfloxacin, in addition to pulsed-field gel electrophoresis patterns with NotI-digested genomic DNA, confirmed nosocomial transmission of infections involving carbapenemase-producing A. baumannii strains.

* Corresponding author. Mailing address: Laboratory of Drug Resistance in Bacteria, Gunma University School of Medicine, 3-39-22, Showa-machi, Maebashi 371-8511, Japan. Phone: 81-27-220-8085. Fax: 81-27-220-8088. E-mail: siyobe{at}akagi.sb.gunma-u.ac.jp.

Journal of Clinical Microbiology, February 2000, p. 526-529, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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