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Journal of Clinical Microbiology, February 2000, p. 570-574, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Ability of the VITEK 2 Advanced Expert System To Identify beta -Lactam Phenotypes in Isolates of Enterobacteriaceae and Pseudomonas aeruginosa

Christine C. Sanders,1 Michel Peyret,2 Ellen Smith Moland,1 Carole Shubert,2 Kenneth S. Thomson,1,* Jean-Marc Boeufgras,3 and W. Eugene Sanders Jr.1

Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska 681781; bioMérieux Inc., Hazelwood, Missouri 630422; and bioMérieux, LaBalme-Les-Grottes, France3

Received 9 August 1999/Returned for modification 22 September 1999/Accepted 9 November 1999

The Advanced Expert System (AES) was used in conjunction with the VITEK 2 automated antimicrobial susceptibility test system to ascertain the beta -lactam phenotypes of 196 isolates of the family Enterobacteriaceae and the species Pseudomonas aeruginosa. These isolates represented a panel of strains that had been collected from laboratories worldwide and whose beta -lactam phenotypes had been characterized by biochemical and molecular techniques. The antimicrobial susceptibility of each isolate was determined with the VITEK 2 instrument, and the results were analyzed with the AES to ascertain the beta -lactam phenotype. The results were then compared to the beta -lactam resistance mechanism determined by biochemical and molecular techniques. Overall, the AES was able to ascertain a beta -lactam phenotype for 183 of the 196 (93.4%) isolates tested. For 111 of these 183 (60.7%) isolates, the correct beta -lactam phenotype was identified definitively in a single choice by the AES, while for an additional 46 isolates (25.1%), the AES identified the correct beta -lactam phenotype provisionally within two or more choices. For the remaining 26 isolates (14.2%), the beta -lactam phenotype identified by the AES was incorrect. However, for a number of these isolates, the error was due to remediable problems. These results suggest that the AES is capable of accurate identification of the beta -lactam phenotypes of gram-negative isolates and that certain modifications can improve its performance even further.

* Corresponding author. Mailing address: Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178. Phone: (402) 280-2921. Fax: (402) 280-1875. E-mail: kstaac{at}creighton.edu.

Journal of Clinical Microbiology, February 2000, p. 570-574, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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