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Journal of Clinical Microbiology, February 2000, p. 591-594, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pig and Goat Blood as Substitutes for Sheep Blood in Blood-Supplemented Agar Media

Chandar Anand,1,2,* Rhonda Gordon,1 Helene Shaw,1 Kevin Fonseca,1,2 and Merle Olsen2

Provincial Laboratory of Public Health, Calgary, Alberta, Canada, T2N 4W4,1 and Department of Microbiology and Infectious Diseases, The University of Calgary, Calgary, Alberta, Canada T2N 4N12

Received 29 July 1999/Returned for modification 28 September 1999/Accepted 1 November 1999

In many developing countries sheep and horse blood, the recommended blood supplements in bacteriological media, are not readily available, whereas pig and goat blood are. Therefore, this study examined the use of pig and goat blood as potential substitutes for sheep blood in blood-supplemented bacteriologic media commonly used in clinical microbiology laboratories. In general, the growth characteristics and colony morphologies of a wide range of aerobic and anaerobic bacteria and Candida albicans were similar on media containing pig, goat, and sheep blood, although differences were found. Enterococcus sp. uniformly produced alpha-hemolysis when incubated in CO2, but in anaerobic conditions the hemolysis varied. In contrast, beta-hemolytic streptococci produced identical hemolytic reactions on all three media. Synergistic hemolysis was not observed on pig blood agar in the CAMP test nor on goat blood agar in the reverse CAMP test. The preparation of chocolate agar (heated) with pig blood required heating to a higher temperature than with sheep or goat blood to yield suitable growth of Haemophilus species. In general, we conclude that pig and goat blood are suitable alternatives to sheep blood for use in bacteriological media in settings where sheep and horse blood are not readily available.

* Corresponding author. Mailing address: Provincial Laboratory of Public Health, 3030 Hospital Dr. NW, Calgary, Alberta, Canada, T2N 4W4. Phone: (403) 670-1201. Fax: (403) 270-2216. E-mail: chandar.anand{at}crha-health.ab.ca.

Journal of Clinical Microbiology, February 2000, p. 591-594, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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