This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by González, L. M.
Right arrow Articles by Garate, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by González, L. M.
Right arrow Articles by Garate, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2000, p. 737-744, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Differential Diagnosis of Taenia saginata and Taenia solium Infection by PCR

Luis Miguel González,1 Estrella Montero,1 Leslie J. S. Harrison,2,* R. Michael E. Parkhouse,3 and Teresa Garate1

Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Centro Nacional de Microbiologia, 28220 Majadahonda, Madrid, Spain1; University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, Scotland EH25 9RG2; and Institute for Animal Health, Pirbright Laboratories, Pirbright, Woking, Surrey, England GU24 0NF3

Received 16 June 1999/Returned for modification 21 July 1999/Accepted 3 November 1999

We have designed species-specific oligonucleotides which permit the differential detection of two species of cestodes, Taenia saginata and Taenia solium. The oligonucleotides contain sequences established for two previously reported, noncoding DNA fragments cloned from a genomic library of T. saginata. The first, which is T. saginata specific (fragment HDP1), is a repetitive sequence with a 53-bp monomeric unit repeated 24 times in direct tandem along the 1,272-bp fragment. From this sequence the two oligonucleotides that were selected (oligonucleotides PTs4F1 and PTs4R1) specifically amplified genomic DNA (gDNA) from T. saginata but not T. solium or other related cestodes and had a sensitivity down to 10 pg of T. saginata gDNA. The second DNA fragment (fragment HDP2; 3,954 bp) hybridized to both T. saginata and T. solium DNAs and was not a repetitive sequence. Three oligonucleotides (oligonucleotides PTs7S35F1, PTs7S35F2, and PTs7S35R1) designed from the sequence of HDP2 allowed the differential amplification of gDNAs from T. saginata, T. solium, and Echinococcus granulosus in a multiplex PCR, which exhibits a sensitivity of 10 pg.

* Corresponding author. Mailing address: University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, Scotland, EH25 9RG. Phone: 44-131-6506217. Fax: 44-131-6506217. E-mail: Leslie.Harrison{at}ed.ac.uk.

Journal of Clinical Microbiology, February 2000, p. 737-744, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nkouawa, A., Sako, Y., Nakao, M., Nakaya, K., Ito, A. (2009). Loop-Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species. J. Clin. Microbiol. 47: 168-174 [Abstract] [Full Text]  
  • Mayta, H., Gilman, R. H., Prendergast, E., Castillo, J. P., Tinoco, Y. O., Garcia, H. H., Gonzalez, A. E., Sterling, C. R., for the Cysticercosis Working Group in Peru, (2008). Nested PCR for Specific Diagnosis of Taenia solium Taeniasis. J. Clin. Microbiol. 46: 286-289 [Abstract] [Full Text]  
  • Yamasaki, H., Allan, J. C., Sato, M. O., Nakao, M., Sako, Y., Nakaya, K., Qiu, D., Mamuti, W., Craig, P. S., Ito, A. (2004). DNA Differential Diagnosis of Taeniasis and Cysticercosis by Multiplex PCR. J. Clin. Microbiol. 42: 548-553 [Abstract] [Full Text]  
  • ABBASI, I., BRANZBURG, A., CAMPOS-PONCE, M., HAFEZ, S. K. A., RAOUL, F., CRAIG, P. S., HAMBURGER, J. (2003). COPRO-DIAGNOSIS OF ECHINOCOCCUS GRANULOSUS INFECTION IN DOGS BY AMPLIFICATION OF A NEWLY IDENTIFIED REPEATED DNA SEQUENCE. Am J Trop Med Hyg 69: 324-330 [Abstract] [Full Text]  
  • Yamasaki, H., Nakao, M., Sako, Y., Nakaya, K., Sato, M. O., Mamuti, W., Okamoto, M., Ito, A. (2002). DNA Differential Diagnosis of Human Taeniid Cestodes by Base Excision Sequence Scanning Thymine-Base Reader Analysis with Mitochondrial Genes. J. Clin. Microbiol. 40: 3818-3821 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»