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Journal of Clinical Microbiology, February 2000, p. 898-901, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Diversity within the VP4 Gene of Rotavirus P[8] Strains: Implications for Reverse Transcription-PCR Genotyping

Miren Iturriza-Gómara,1 Jon Green,2 David W. G. Brown,2 Ulrich Desselberger,1 and James J. Gray1,*

Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge CB2 2QW,1 and Enteric and Respiratory Virus Laboratory, Virus Reference Division, Central Public Health Laboratory, Colindale, London NW9 5HT,2 United Kingdom

Received 28 June 1999/Returned for modification 20 September 1999/Accepted 1 November 1999

A degenerate version (1T1-D) of the rotavirus P[8]-specific primer (1T-1) allowed strains previously untypeable due to the accumulation of point mutations at the primer binding site to be P typed by reverse transcription-PCR. Sequencing of the cDNA followed by sequence alignment and phylogenetic analysis identified lineages and sublineages within the rotavirus P[8] types, while the use of 1T-1 or 1T-1D primers did not yield viral clusters in any particular lineage.

* Corresponding author. Mailing address: Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Hills Rd., Cambridge CB2 2QW, United Kingdom. Phone: 44-1223-257028. Fax: 44-1223-242775. E-mail: jg2{at}mole.bio.cam.ac.uk.

Journal of Clinical Microbiology, February 2000, p. 898-901, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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