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Journal of Clinical Microbiology, March 2000, p. 1023-1031, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Prevalence and Characterization of Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Food, and Children during a One-Year Prospective Study in France

Nathalie Pradel,1 Valérie Livrelli,1,* Christophe De Champs,2 Jean-Bernard Palcoux,3 Alain Reynaud,4 Flemming Scheutz,5 Jacques Sirot,2 Bernard Joly,1 and Christiane Forestier1

Groupe de Recherche Pathogénie Bactérienne Intestinale, Faculté de Pharmacie, Université d'Auvergne Clermont-1,1 Laboratoire de Bactériologie, Faculté de Médecine,2 Service de Pédiatrie, Centre Hospitalier Universitaire,3 and Laboratoire Départemental d'Analyses Vétérinaires et Biologiques,4 Clermont-Ferrand, France, and The International Escherichia Centre (WHO), Statens Serum Institut, Copenhagen, Denmark5

Received 20 September 1999/Returned for modification 1 November 1999/Accepted 20 December 1999

During a 1-year survey of Shiga toxin-producing Escherichia coli (STEC) prevalence in central France, 2,143 samples were investigated by PCR for Shiga toxin-encoding genes. A total of 330 (70%) of 471 fecal samples collected from healthy cattle at the Clermont-Ferrand slaughterhouse, 47 (11%) of 411 beef samples, 60 (10%) of 603 cheese samples, and 19 (3%) of 658 stool specimens from hospitalized children with and without diarrhea were positive for the stx gene(s). A STEC strain was isolated from 34% (162 of 471) of bovine feces, 4% (16 of 411) of beef samples, 1% (5 of 603) of cheese samples, and 1.5% (10 of 658) of stool specimens. Of the 220 STEC strains isolated, 34 (15%) harbored the stx1 gene, 116 (53%) harbored the stx2 gene, and 70 (32%) carried both the stx1 and stx2 genes. However, 32 (14.5%) were not cytotoxic for Vero cells. The eae gene, found in 12 (5%) of the 220 strains, was significantly associated with the stx1 gene and with isolates from children. Sequences homologous to ehxA were found in 102 (46%) of the 220 strains. Thirteen serotypes, OX3:H2, O113:H21, O113:H4, OX3:H21, O6:H10, OX178:H19, O171:H2, O46:H38, O172:H21, O22:H16, O91:H10, O91:H21, and O22:H8, accounted for 102 (55%) of 186 typeable isolates, and only one strain (0.5% of the 186 STEC isolates from cattle), belonged to the O157:H7 serotype. We showed that the majority of the STEC isolates from cattle, beef, and cheese were not likely to be pathogenic for humans and that the STEC strains isolated from children in this study were probably not responsible for diarrheal disease. Finally, the strains associated with hemolytic-uremic syndrome in the same geographical area were shown to belong to particular subsets of the STEC population found in the bovine reservoir.

* Corresponding author. Mailing address: Groupe de Recherche Pathogénie Bactérienne Intestinale, Université d'Auvergne, Faculté de Pharmacie, 28 Place Henri Dunant, 63 001 Clermont-Ferrand, France. Phone: (33) 473 60 80 19. Fax: (33) 473 27 74 94. E-mail: Valerie.Livrelli{at}u-clermont1.fr.

Journal of Clinical Microbiology, March 2000, p. 1023-1031, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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