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Journal of Clinical Microbiology, March 2000, p. 1180-1183, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Characterization of Cryptosporidium Isolates Obtained from Human Immunodeficiency Virus-Infected Individuals Living in Switzerland, Kenya, and the United States

Una Morgan,1,* Rainer Weber,2 Lihua Xiao,3 Irshad Sulaiman,3 R. C. Andrew Thompson,1 Wangeci Ndiritu,4 Altaf Lal,3 Anne Moore,3 and Peter Deplazes5

World Health Organisation Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and State Agricultural Biotechnology Centre, Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia1; Division of Infectious Diseases, University Hospital, CH-8091 Zurich,2 and Institute of Parasitology, University of Zurich, CH-8057 Zurich,5 Switzerland; Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303413; and Kenya Medical Research Institute, Nairobi, Kenya4

Received 27 September 1999/Returned for modification 1 December 1999/Accepted 21 December 1999

A total of 22 Cryptosporidium isolates from human immunodeficiency virus-infected patients from Kenya, Switzerland, and the United States were examined at three genetic loci: the 18S ribosomal DNA, HSP-70, and acetyl coenzyme A synthetase genes. Four distinct Cryptosporidium genotypes were identified: (i) the Cryptosporidium parvum "human" genotype, (ii) the C. parvum "cattle" genotype, (iii) Cryptosporidium felis, and (iv) Cryptosporidium meleagridis. This is the first report of C. meleagridis in a human host. These results and those of others indicate that immunocompromised individuals are susceptible to a wide range of Cryptosporidium species and genotypes. Future studies are required to understand the full public health significance of Cryptosporidium genotypes and species in immunocompromised hosts.

* Corresponding author. Mailing address: Murdoch University, Murdoch, WA 6150, Australia. Phone: (08) 9360 2457. Fax: (08) 9310 4144. E-mail: morgan{at}numbat.murdoch.edu.au.

Journal of Clinical Microbiology, March 2000, p. 1180-1183, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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