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Journal of Clinical Microbiology, March 2000, p. 1241-1243, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Development of a Hypersensitive Detection Method for Human Parvovirus B19 DNA

Kazuaki Sato,1,* Eiji Matsuda,1 Keiichi Kamisango,1 Hiroaki Iwasaki,1 Shuzo Matsubara,1 and Yasuko Matsunaga2

Chugai Pharmaceutical Co. Ltd., Central Research Laboratories, 3-41-8 Takada, Toshima-ku, Tokyo 171-8545,1 and National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640,2 Japan

Received 5 August 1999/Returned for modification 3 November 1999/Accepted 17 December 1999

A new detection method for human parvovirus B19 DNA was established using PCR coupled with a hybridization protection assay. The amplified product was detected using acridinium ester-labeled DNA probes. By this method, a few copies of B19 DNA were detected in human serum albumin.

* Corresponding author. Mailing address: Chugai Pharmaceutical Co. Ltd., 3-41-8 Takada, Toshima-ku, Tokyo 171-8545, Japan. Phone: 81-3-3987-7196. Fax: 81-3-3989-0785. E-mail: satokza{at}chugai-pharm.co.jp.

Journal of Clinical Microbiology, March 2000, p. 1241-1243, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Daly, P., Corcoran, A., Mahon, B. P., Doyle, S. (2002). High-Sensitivity PCR Detection of Parvovirus B19 in Plasma. J. Clin. Microbiol. 40: 1958-1962 [Abstract] [Full Text]  
  • Harder, T. C., Hufnagel, M., Zahn, K., Beutel, K., Schmitt, H.-J., Ullmann, U., Rautenberg, P. (2001). New LightCycler PCR for Rapid and Sensitive Quantification of Parvovirus B19 DNA Guides Therapeutic Decision-Making in Relapsing Infections. J. Clin. Microbiol. 39: 4413-4419 [Abstract] [Full Text]  


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