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Journal of Clinical Microbiology, March 2000, p. 992-995, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Analysis of CAP59 Gene Sequences from Five Serotypes of Cryptococcus neoformans

Yuka Nakamura,1,* Rui Kano,2 Shinichi Watanabe,1 and Atsuhiko Hasegawa2

Department of Dermatology, Teikyo University School of Medicine, Tokyo,1 and Department of Pathobiology, Nihon University School of Veterinary Medicine, Kanagawa,2 Japan

Received 9 August 1999/Returned for modification 4 November 1999/Accepted 21 December 1999

The nucleotide sequences of CAP59 genes from five serotypes of Cryptococcus neoformans were analyzed for their phylogenetic relationships. Approximately 600-bp genomic DNA fragments of the CAP59 gene were amplified from each isolate by PCR and sequenced. The CAP59 nucleotide sequences of C. neoformans showed more than 90% similarity among the five serotypes. By phylogenetic analysis, their sequences were divided into three clusters: serotypes A and AD, serotypes B and C, and serotype D. In addition, the results of reduced amino acid sequences were similar to the nucleotide sequence data. These data revealed that serotype AD was genetically close to serotype A rather than serotype D, although it had been considered to be a mixed type of serotype A and D by serological analysis. Furthermore, the nucleotide sequences of the serotype B and C isolates of C. neoformans were very similar to each other. These results indicated that serotype B and C isolates belonging to C. neoformans var. gattii were genetically homogeneous and closely related. The molecular analysis of the CAP59 gene will provide useful information for the differentiation of serotypes of C. neoformans and for an understanding of their phylogenetic relationships.

* Corresponding author. Mailing address: Department of Dermatology, Teikyo University School of Medicine, 11-1 Kaga-2, Itabashi-ku, Tokyo 173-8605, Japan. Phone: 81-3-3964-1211, ext. 1636. Fax: 81-3-5375-5314. E-mail: yuka{at}med.teikyo-u.ac.jp.

Journal of Clinical Microbiology, March 2000, p. 992-995, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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