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Journal of Clinical Microbiology, April 2000, p. 1623-1627, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Fluorescent Amplified-Fragment Length Polymorphism Genotyping of Salmonella enterica subsp. enterica Serovars and Comparison with Pulsed-Field Gel Electrophoresis Typing

Bjørn-Arne Lindstedt,1,* Even Heir,1 Traute Vardund,1 and Georg Kapperud1,2

Department of Bacteriology, National Institute of Public Health, N-0403 Oslo,1 and Department of Pharmacology, Microbiology, and Food Hygiene, Norwegian College of Veterinary Medicine, N-0033 Oslo,2 Norway

Received 15 September 1999/Returned for modification 28 December 1999/Accepted 31 January 2000

We have performed the fluorescently labeled amplified-fragment length polymorphism (FAFLP) method on 97 strains of the food-borne pathogen Salmonella enterica subsp. enterica comprising seven different serovars using the restriction enzymes EcoRI and MseI. From the total FAFLP fingerprinted strains, 81 were compared with pulsed-field gel electrophoresis (PFGE) typing of the same strains. The FAFLP method showed a discriminatory power equal to that of PFGE. We report a fast, robust, and high-resolution adaptation of the AFLP assay for fingerprinting S. enterica subsp. enterica serovars with capillary electrophoresis that can be scaled to high throughput on automated analysis instruments.

* Corresponding author. Mailing address: Department of Bacteriology, National Institute of Public Health, Geitmyrsveien 75, P.O. Box 4404 Torshov, N-0403 Oslo, Norway. Phone: 47 22042200. Fax: 47 22042518. E-mail: bjorn-arne.lindstedt{at}folkehelsa.no.

Journal of Clinical Microbiology, April 2000, p. 1623-1627, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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