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Journal of Clinical Microbiology, April 2000, p. 1645-1647, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of a Commercial Enzyme-Linked Immunosorbent Assay with Immunofluorescence and Complement Fixation Tests for Detection of Coxiella burnetii (Q Fever) Immunoglobulin M

Peter R. Field,1 Jody L. Mitchell,2 Avelina Santiago,1 David J. Dickeson,1 Sau-Wan Chan,1 David W. T. Ho,1 Alan M. Murphy,3 Andrea J. Cuzzubbo,2 and Peter L. Devine2,*

Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead,1 PanBio, Brisbane,2 and Viral Diagnostic and Referral Laboratory, North Ryde,3 Australia

Received 6 August 1999/Returned for modification 17 October 1999/Accepted 3 January 2000

A commercially available enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Q fever (PanBio Coxiella burnetii immunoglobulin M [IgM] ELISA, QFM-200) was compared to the indirect fluorescent antibody test (IFAT) for C. burnetii IgM and the complement fixation test (CFT). The ELISA demonstrated 92% agreement with the reference method (IFAT), and gave a sensitivity of 99% (69 of 70 samples) and a specificity of 88% (106 of 121). Specificity can be increased with confirmation by IFAT. CFT was found to have a specificity of 90% (107 of 119), although it was lacking in sensitivity (73%; 51 of 70). No cross-reactivity was observed in the ELISA with serum samples from patients with mycoplasma (n = 6), chlamydia (n = 5), or legionella (n = 4) infections, although 2 of 5 patients with leptospirosis and 1 of 4 samples containing rheumatoid factor (RF) demonstrated positive results in the ELISA. Results indicate that the performance of the PanBio C. burnetii (Q fever) IgM ELISA (F = 187) is superior to that of CFT (F = 163), and consequently the ELISA should be a useful aid in the diagnosis of acute Q fever.

* Corresponding author. Mailing address: PanBio Pty., Ltd., 116 Lutwyche Rd., Windsor 4030, Queensland, Australia. Phone: 61-7-33571177. Fax: 61-7-33571222. E-mail: peter_devine{at}panbio.com.au.

Journal of Clinical Microbiology, April 2000, p. 1645-1647, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Setiyono, A., Ogawa, M., Cai, Y., Shiga, S., Kishimoto, T., Kurane, I. (2005). New Criteria for Immunofluorescence Assay for Q Fever Diagnosis in Japan. J. Clin. Microbiol. 43: 5555-5559 [Abstract] [Full Text]  
  • Thorpe, R., Swanson, S. J (2005). Current Methods for Detecting Antibodies against Erythropoietin and Other Recombinant Proteins. CVI 12: 28-39 [Full Text]  
  • Field, P. R., Santiago, A., Chan, S.-W., Patel, D. B., Dickeson, D., Mitchell, J. L., Devine, P. L., Murphy, A. M. (2002). Evaluation of a Novel Commercial Enzyme-Linked Immunosorbent Assay Detecting Coxiella burnetii-Specific Immunoglobulin G for Q Fever Prevaccination Screening and Diagnosis. J. Clin. Microbiol. 40: 3526-3529 [Abstract] [Full Text]  


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