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Journal of Clinical Microbiology, April 2000, p. 1651-1655, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Typing and Exopolysaccharide Biosynthesis of Burkholderia cepacia Isolates from a Portuguese Cystic Fibrosis Center

João A. Richau,1 Jorge H. Leitão,1 Manuela Correia,1 Luís Lito,2 Maria José Salgado,2 Celeste Barreto,3 Paola Cescutti,4 and Isabel Sá-Correia1,*

Centro de Engenharia Biológica e Química, Instituto Superior Técnico, 1049-001 Lisbon,1 and Laboratório de Bacteriologia2 and Departamento de Fibrose Quística,3 Hospital de Santa Maria, 1500 Lisbon, Portugal, and Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, I-34127 Trieste, Italy4

Received 19 July 1999/Returned for modification 15 October 1999/Accepted 7 January 2000

This work describes the first epidemiological survey of Burkholderia cepacia involved in pulmonary infections among the Portuguese population with cystic fibrosis (CF) who attended the major CF treatment Center in Lisbon at Sta. Maria Hospital from 1995 to the end of 1997. The characterization of the genomic relatedness of the isolates was based on the analysis of their ribopatterns (with EcoRI) followed by construction of a ribotype-based phylogenetic tree. This study was complemented with macrorestriction fragment analysis by pulsed-field gel electrophoresis. After optimization of the solid growth medium, we found that exopolysaccharide (EPS) production by B. cepacia CF isolates is not as rare a phenomenon as was thought before; indeed, 70% of the isolates examined were EPS producers.

* Corresponding author. Mailing address: Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. Phone: 351-218417233. Fax: 351-218480072. E-mail: pcisc{at}alfa.ist.utl.pt.

Journal of Clinical Microbiology, April 2000, p. 1651-1655, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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