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Journal of Clinical Microbiology, April 2000, p. 1656-1660, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of an Epitope Common to Genogroup 1 "Norwalk-Like Viruses"

Antony D. Hale,1,2 Tomoyuki N. Tanaka,3 Noritoshi Kitamoto,4 Max Ciarlet,1 Xi Jiang,5 Naokazu Takeda,6 David W. G. Brown,2 and Mary K. Estes1,*

Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 770301; Center for Pediatric Research, Eastern Virginia Medical School, Norfolk, Virginia 235105; Enteric and Respiratory Virus Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom2; and Department of Microbiology, Wakayama Medical College, Wakayama,3 School of Humanities for Environmental Policy and Technology, Himeji Institute of Technology, Himeji, Hyogo,4 and Department of Virology II, National Institute of Infectious Diseases, Tokyo,6 Japan

Received 19 October 1999/Returned for modification 24 December 1999/Accepted 31 January 2000

A panel of 10 monoclonal antibodies (MAbs) to recombinant Norwalk virus (NV) capsid protein were tested in competition enzyme-linked immunosorbent assays. Patterns of competition indicated that these MAbs recognize six to eight epitopes covering five nonoverlapping regions of the capsid protein. A single epitope, recognized by NV MAbs NV3901, NV3912, and NV2461 was found to occur in the majority of genogroup 1 (G1) but not genogroup 2 (G2) "Norwalk-like viruses" (NLVs). This observation supports the subdivision of human NLVs into two genogroups and provides an assay for the rapid identification of G1 NLVs in fecal specimens.

* Corresponding author. Mailing address: Division of Molecular Virology, Baylor College of Medicine, One Baylor Plaza, Room 923E, Mailstop BCM-385, Houston, TX 77030. Phone: (713) 798-3585. Fax: (713) 798-3586. E-mail: mestes{at}bcm.tmc.edu.

Journal of Clinical Microbiology, April 2000, p. 1656-1660, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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